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  2015 Fearchar Maclennan: Raasay

  Clayton, Ian; Carnie, Andrew; University of Nevada, Reno; University of Arizona; Muriel Fisher, Andrew Carnie, Ian Clayton, Fearchar Maclennan
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  Comparison of the Transcriptomes and Proteomes of Serum Exosomes from Marek's Disease Virus-Vaccinated and Protected and Lymphoma-Bearing Chickens

  Neerukonda, Sabari Nath; Tavlarides-Hontz, Phaedra; McCarthy, Fiona; Pendarvis, Kenneth; Parcells, Mark S; Univ Arizona, Dept Anim & Comparat Biomed Sci (MDPI, 2019-02-05)

  Marek's disease virus (MDV) is the causative agent of Marek's disease (MD), a complex pathology of chickens characterized by paralysis, immunosuppression, and T-cell lymphomagenesis. MD is controlled in poultry production via vaccines administered in ovo or at hatch, and these confer protection against lymphoma formation, but not superinfection by MDV field strains. Despite vaccine-induced humoral and cell-mediated immune responses, mechanisms eliciting systemic protection remain unclear. Here we report the contents of serum exosomes to assess their possible roles as indicators of systemic immunity, and alternatively, tumor formation. We examined the RNA and protein content of serum exosomes from CVI988 (Rispens)-vaccinated and protected chickens (VEX), and unvaccinated tumor-bearing chickens (TEX), via deep-sequencing and mass spectrometry, respectively. Bioinformatic analyses of microRNAs (miRNAs) and predicted miRNA targets indicated a greater abundance of tumor suppressor miRNAs in VEX compared to TEX. Conversely, oncomiRs originating from cellular (miRs 106a-363) and MDV miRNA clusters were more abundant in TEX compared to VEX. Most notably, mRNAs mapping to the entire MDV genome were identified in VEX, while mRNAs mapping to the repeats flanking the unique long (IRL/TRL) were identified in TEX. These data suggest that long-term systemic vaccine-induced immune responses may be mediated at the level of VEX which transfer viral mRNAs to antigen presenting cells systemically. Proteomic analyses of these exosomes suggested potential biomarkers for VEX and TEX. These data provide important putative insight into MDV-mediated immune suppression and vaccine responses, as well as potential serum biomarkers for MD protection and susceptibility.
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  Construction and Optimization of Through-Hole LED Models for Use in Designing Traffic Signboards

  Lee, Ted; Chen, Yi-Chun; Univ Arizona, Coll Opt Sci (MDPI, 2019-02)

  A modeling procedure was proposed for the through-hole LEDs commonly used in traffic signs. The measurements from a sample batch of LEDs were performed at several midfield distances to provide a set of representative angular intensity distributions as the target pattern in the modeling process. The flat outer dimensions of the LED were accurately measured while the curvature and refractive index of the packaging dome were roughly estimated. These physical parameters were used to build a preliminary LED model in the Monte Carlo simulation software. The simulated angular intensity distribution at each distance was generated by tracing 20,000,000 rays. The normalized cross correlation (NCC) between the measured and simulated data was calculated to represent the resemblance of the model to the real LED. The roughly estimated parameters were then varied within their physical limits to optimize the NCC value. The possibilities of two parameters having interactions were also considered. The final model has all NCCs above 98.8% between the target and simulated patterns.
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  Allergic Contact Dermatitis to Mastisol Adhesive Used for Skin Closure in Orthopedic Surgery: A Case Report

  Ezeh, Ugonna E; Price, Harper N; Belthur, Mohan V; Univ Arizona, Dept Child Hlth, Coll Med (LIPPINCOTT WILLIAMS & WILKINS, 2018-09-01)

  We report on a rare case of allergic contact dermatitis (ACD) from Mastisol liquid adhesive. We are aware of a few reports in the medical literature, but none describes an allergic reaction during the third exposure to the offending agent. Our patient was a 20-year-old Caucasian man with a history of cerebral palsy spastic hemiplegia who underwent single-event multilevel soft-tissue surgery to optimize function of his left upper extremity. He developed a severe cutaneous allergic reaction after his third exposure to Mastisol. He was subsequently admitted to the inpatient service and managed without further complications by a multidisciplinary team comprising orthopedics, pediatrics, and dermatology. We discuss the etiology, clinical features, diagnosis, and treatment of this entity, and we also review relevant available literature on the subject. We aim at creating further awareness of allergic reactions because of exposure to available skin-prepping and wound-dressing agents.
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  Transdisciplinarity across two-tiers The case of applied linguistics and literary studies in U.S. foreign language departments

  Warner, Chantelle; Univ Arizona (JOHN BENJAMINS PUBLISHING CO, 2018)

  In the ten years since the Modern Language Association published their report, "Foreign Languages and Higher Education: New Structures for a Changed World" (2007) dissatisfaction with the "two-tiered configuration" of US foreign language departments has become increasingly vocal. While the target of the criticism is often the curriculum, it has often been noted that programmatic bifurcations mirror institutional hierarchies, e.g. status differences between specialists in literary and cultural studies and experts in applied linguistics and language pedagogy (e.g. Maxim et al., 2013; Allen & Maxim, 2012). This chapter looks at the two-tiered structure of collegiate modern language departments from the perspectives of the transdisciplinary shape-shifters who maneuver within them - scholars working between applied linguistics and literary studies. These individuals must negotiate the methodologies and the institutional positions available to them - in many instances, the latter is what has prompted them to work between fields in the first place. The particular context of US foreign language and literature departments serves as a case study of the lived experiences of doing transdisciplinary work in contexts that are characterized by disciplinary hierarchies and the chapter ends with a call for applied linguistics to consider not only the epistemic, but also the institutional and affective labor needed to sustain transdisciplinary work.

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