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    Background and Research on Copper Alloys as an Antimicrobial Surface and the CusB protein of the Copper-Transporting Efflux System CusCFBA

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    azu_etd_mr20100071_sip1_m.pdf
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    Author
    Andrade, Cassandra Jo
    Issue Date
    2010-05
    
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    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    Bactericidal properties of copper surfaces have been investigated in search of selfsanitizing materials in food and healthcare industries. However, bacteria in these environments are rapidly acquiring antibiotic and heavy metal resistance, which is thought to be a co-selection process (Baker-Austin, 2006). Copper-resistant strains of Escherichia coli and Enterococcus faecium isolated from pigs fed copper sulfate were examined (Hasman, 2002). Survival of strains was tested by timed incubation on onesquare- inch copper alloys with varying degrees of moisture during inoculation of the surfaces. Results showed rapid killing of E. coli and E. faecium copper-resistant strains when samples were spread in a thin layer on alloys with 85 % or greater copper content. E. coli strains had short survival rates under dry conditions while E. faecium strains were less affected. Dry or wet inoculations had no effect on the survival rates on stainless steel, since strains survived equally and no die-off was seen. Re-inoculation with E. coli on the alloys every 3 hours over a 24-hour period showed no CFUs remaining at each time point tested while bacteria survived without reduction of CFUs on the stainless steel controls after 24 hours. Results indicate that the bactericidal properties of metallic copper surfaces can be effective in killing copper-resistant strains of E. coli and E. faecium.
    Type
    text
    Electronic Thesis
    Degree Name
    B.S.
    Degree Level
    bachelors
    Degree Program
    Honors College
    Microbiology
    Degree Grantor
    University of Arizona
    Collections
    Honors Theses

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