The Role of the Rck1 and Rck2 Kinases in the Osmotic Stress Response of Budding Yeast
| dc.contributor.author | Blaire, Rachael Haley | |
| dc.creator | Blaire, Rachael Haley | en_US |
| dc.date.accessioned | 2011-10-24T16:42:58Z | |
| dc.date.available | 2011-10-24T16:42:58Z | |
| dc.date.issued | 2010-05 | |
| dc.identifier.citation | Blaire, Rachael Haley. (2010). The Role of the Rck1 and Rck2 Kinases in the Osmotic Stress Response of Budding Yeast (Bachelor's thesis, University of Arizona, Tucson, USA). | |
| dc.identifier.uri | http://hdl.handle.net/10150/146568 | |
| dc.description.abstract | In response to high extracellular salt levels, Saccharomyces cerevisiae activates the HOG1 mitogen-activated protein kinase (MAPK) cascade, which signals to decrease cell growth and provide a method of survival in osmotic stress. Studies have shown that this occurrence can be attributed to an interaction between Hog1 and TOR (target of rapamycin); however, the method of interaction is unknown. Here, we use microarrays to investigate Rck2, a direct downstream substrate of Hog1, and its close homologue Rck1, as possible intermediates connecting Hog1 and TOR in the osmotic stress response. Our findings demonstrated that Rck1 and Rck2 were not acting in a salt dependent manner; however knocking out these kinases did cause significant changes in gene expression at 2452 genes. Rather, using a gene ontology search program, we found that Rck1 and Rck2 are involved in regulating cell growth, and that they interact with 7 transcription factors: Fhl1, Rap1, Ino2, Hir1, Hir2, Spt2 and YML081W. | |
| dc.language.iso | en | en_US |
| dc.publisher | The University of Arizona. | en_US |
| dc.rights | Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. | en_US |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
| dc.title | The Role of the Rck1 and Rck2 Kinases in the Osmotic Stress Response of Budding Yeast | en_US |
| dc.type | text | en_US |
| dc.type | Electronic Thesis | en_US |
| thesis.degree.grantor | University of Arizona | en_US |
| thesis.degree.level | bachelors | en_US |
| thesis.degree.discipline | Honors College | en_US |
| thesis.degree.discipline | Molecular and Cellular Biology | en_US |
| thesis.degree.name | B.S. | en_US |
| refterms.dateFOA | 2018-05-27T19:21:24Z | |
| html.description.abstract | In response to high extracellular salt levels, Saccharomyces cerevisiae activates the HOG1 mitogen-activated protein kinase (MAPK) cascade, which signals to decrease cell growth and provide a method of survival in osmotic stress. Studies have shown that this occurrence can be attributed to an interaction between Hog1 and TOR (target of rapamycin); however, the method of interaction is unknown. Here, we use microarrays to investigate Rck2, a direct downstream substrate of Hog1, and its close homologue Rck1, as possible intermediates connecting Hog1 and TOR in the osmotic stress response. Our findings demonstrated that Rck1 and Rck2 were not acting in a salt dependent manner; however knocking out these kinases did cause significant changes in gene expression at 2452 genes. Rather, using a gene ontology search program, we found that Rck1 and Rck2 are involved in regulating cell growth, and that they interact with 7 transcription factors: Fhl1, Rap1, Ino2, Hir1, Hir2, Spt2 and YML081W. |
