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    The Effect of Prenatal Nicotine Exposure on Ventilation Parameters and Receptor Expression in the Neonatal Rat Brainstem

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    Author
    Saccomano, Margaret Eloise
    Issue Date
    2010-05
    
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    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    Prenatal nicotine exposure has recently been linked to respiratory dysfunction in infants and has been named one of the largest contributing factors in Sudden Infant Death Syndrome. Experiments were performed using the neonatal rat model to determine the mechanism behind the link between prenatal nicotine exposure and respiratory dysfunction, clinically manifesting itself as SIDS. Protocol called for full body plethysmography to be performed on nicotine exposed or saline exposed neonates on postnatal days 1, 3, 6, 9, 12, 18 and 21. However, a dysfunctioning apparatus impeded ventilation procedures. Animals were then transcardially perfused, postfixed and brains were dissected. Medullas were cut into 40 μm transverse slices and mounted on electrostatic slides. Immunohistochemistry was used to fluorescently tag cells containing important structure and receptors within the medulla including NeuN, NK-1R, nAChR, GABA-AR and Glutamate AMPAR. Cells were visualized for receptor expression and receptor density in control animals vs. PNE animals was studied. Needed changes in protocol prevented conclusive findings that established the relationship in question. These changes included the use of Trizma buffer over PBS and the reduction in the concentration of the fluorescent secondary antibodies used. However these changes will prove to be useful information in further experiments.
    Type
    text
    Electronic Thesis
    Degree Name
    B.S.
    Degree Level
    bachelors
    Degree Program
    Honors College
    Physiology
    Degree Grantor
    University of Arizona
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    Honors Theses
    Honors Theses

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