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dc.contributor.authorSinha, Natasha
dc.creatorSinha, Natashaen_US
dc.date.accessioned2011-10-25T18:40:34Z
dc.date.available2011-10-25T18:40:34Z
dc.date.issued2010-08
dc.identifier.urihttp://hdl.handle.net/10150/146930
dc.description.abstractThe establishment of polarity in epithelial cells is dependent on the proper distribution of polarity proteins to the apical or basolateral domain. In addition, these cells are dependent on the necessary trafficking of junctional proteins to cells junctions for cell-cell interactions. Rab GTPases are involved in a number of membrane trafficking pathways in the cell. Here we show Rab14's role in regulating the establishment of polarity and junction formation in epithelial cells. Cyst culture was used to show inactive-Rab14 affects single lumen formation and targeting of gp135, an apically targeted protein, thus showing Rab14 may regulate epithelial polarity. In addition, calcium-switch experiments were used to show that not only do cells expressing inactive Rab14 take longer to lose their cell-to-cell contact, but they also regain their contacts more quickly, indicating Rab14 may be playing a role in junction formation. This was further supported by immunostaining experiments examining the distribution of junctional proteins; increased amounts of these proteins at the junction in inactive-Rab14 at the end-point showed that Rab14 may be involved in endocytosis or recycling of junctional components.
dc.language.isoenen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.titleRab14: Role in Cell Polarity and Junction Formationen_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.levelbachelorsen_US
thesis.degree.disciplineHonors Collegeen_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.nameB.S.en_US
refterms.dateFOA2018-08-22T11:23:02Z
html.description.abstractThe establishment of polarity in epithelial cells is dependent on the proper distribution of polarity proteins to the apical or basolateral domain. In addition, these cells are dependent on the necessary trafficking of junctional proteins to cells junctions for cell-cell interactions. Rab GTPases are involved in a number of membrane trafficking pathways in the cell. Here we show Rab14's role in regulating the establishment of polarity and junction formation in epithelial cells. Cyst culture was used to show inactive-Rab14 affects single lumen formation and targeting of gp135, an apically targeted protein, thus showing Rab14 may regulate epithelial polarity. In addition, calcium-switch experiments were used to show that not only do cells expressing inactive Rab14 take longer to lose their cell-to-cell contact, but they also regain their contacts more quickly, indicating Rab14 may be playing a role in junction formation. This was further supported by immunostaining experiments examining the distribution of junctional proteins; increased amounts of these proteins at the junction in inactive-Rab14 at the end-point showed that Rab14 may be involved in endocytosis or recycling of junctional components.


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