PublisherThe University of Arizona.
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AbstractVascular endothelial growth factor B (VEGF-B) has shown potential for therapeutic implementation as a neuroprotective factor in the treatment of neurodegenerative disorders, including Parkinson's disease (PD). The goals of this study were two-fold: (i) to determine the neurotrophic effect of both known VEGF-B isoforms on primary neurite length of dopaminergic (DA) neurons in culture, and (ii) to compare this effect against the neurotrophic effects of other growth factors known to increase neurite outgrowth in culture. Glial-derived neurotrophic factor (GDNF), vascular endothelial growth factor A (VEGF-A165), pigment epithelium derived factor (PEDF), VEGF-B186, and VEGF-B167 were added individually to primary midbrain cultures at various concentrations, with untreated midbrain cultures serving as controls. As expected, GDNF, PEDF, and VEGF-A165 all had a significant effect on primary neurite outgrowth at one or more of their tested concentrations (1ng/mL; 5ng/mL; 1ng/mL and 5ng/mL, respectively) relative to control measurements. VEGF-B186 was shown to have a significant effect on primary neurite outgrowth at all three tested concentrations (5ng/mL; 10 ng/mL; 20 ng/mL), while VEGF-B167 showed a strong neurotrophic trend at both concentrations (5ng/mL; 10ng/mL) that failed to reach significance. Curiously, the effects of GDNF, PEDF, and VEGF-A165 on neurite outgrowth were not as pronounced as those previously observed. However, comparison between both VEGF-B isoforms and the other factors suggests a comparable neurotrophic capacity. We conclude that the growth factor VEGF-B186 increases primary neurite outgrowth of dopaminergic neurons in culture.
Degree ProgramHonors College
Biochemistry and Molecular Biophysics