Translational Regulation of ICP22 on Other Viral Genes
Publisher
The University of Arizona.Rights
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.Abstract
The purpose of this experiment was to observe ICP22's effects on expression of immediate early, early, and late genes of herpes simplex virus-1. This was tested through GFP fluorescence of reporter plasmids after infection with the KOS wild type or the n199 ICP22 null virus. ICP22 suppressed the activity of six of the eight promoters tested, which were ICPO, ICP22/47, ICP27, TK, gC, and VP5. The two viral promoters that did not show suppression were ICP4 and ICP6. We conclude that ICP22 is not a universal repressor, but rather specifically targets certain viral genes and directly regulates them.Type
textElectronic Thesis
Degree Name
B.S.Degree Level
bachelorsDegree Program
Honors CollegeMolecular and Cellular Biology