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dc.contributor.advisorDibbs, Hyatten_US
dc.contributor.authorWang, Xiao-yu.
dc.creatorWang, Xiao-yu.en_US
dc.date.accessioned2011-10-31T17:05:19Z
dc.date.available2011-10-31T17:05:19Z
dc.date.issued1988en_US
dc.identifier.urihttp://hdl.handle.net/10150/184329
dc.description.abstractMicrovitellogenin is a female specific yolk protein from the tobacco hornworm moth Manduca sexta. A cDNA library was constructed from poly (A)⁺ RNA isolated from adult female fat body. cDNA clones of mRNA for microvitellogenin were isolated by screening the cDNA library with antiserum against microvitellogenin. The results of Northern blot analysis and hybrid selection indicated that the cDNA clone was specific for microvitellogenin. The complete nucleotide sequence of the 834 base pair cDNA insert has been determined by the dideoxy chain termination method. The deduced amino acid sequence was compared with the N-terminal sequence determined by Edman degradation, an amino terminal extension of 17 amino acids appeared to be a signal peptide. The cDNA sequence predicts that the mature microvitellogenin is a protein of 232 amino acids with a calculated molecular weight of 26,201. A comparison of the translated amino acid sequence with the sequences in National Biomedical Research Foundation protein library did not establish any sequence similarity with known proteins. The microvitellogenin gene begins to be expressed in the fat body on the first day of the wandering (prepupal) females as determined by using the cDNA insert as a probe to hybridize with the mRNA for microvitellogenin. The cDNA probe was also used to screen a genomic library of M. sexta, yielding three genomic clones for microvitellogenin. One of them was characterized and it contained the complete microvitellogenin gene. The gene sequence was determined. Comparison to the cDNA sequence showed that the microvitellogenin gene contains an intron near the 5'-end of the non-coding region. The 5'-flanking sequence of the gene has been compared to the same regions of yp genes of Drosophila and vitellogenin genes of locust, some similar sequences have been observed and discussed.
dc.language.isoenen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.subjectProteins -- Analysis.en_US
dc.subjectAmino acid sequence.en_US
dc.subjectTobacco hornworm -- Eggs.en_US
dc.titleDeduced amino acid sequence and gene sequence of microvitellogenin, a female specific hemolymph and egg protein from the tobacco hornworm, Manduca sexta.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.identifier.oclc701103926en_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.leveldoctoralen_US
dc.contributor.committeememberWells, Michael A.en_US
dc.identifier.proquest8809948en_US
thesis.degree.disciplineBiochemistryen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.namePh.D.en_US
dc.description.noteThis item was digitized from a paper original and/or a microfilm copy. If you need higher-resolution images for any content in this item, please contact us at repository@u.library.arizona.edu.
dc.description.admin-noteOriginal file replaced with corrected file July 2023.
refterms.dateFOA2018-08-22T17:27:37Z
html.description.abstractMicrovitellogenin is a female specific yolk protein from the tobacco hornworm moth Manduca sexta. A cDNA library was constructed from poly (A)⁺ RNA isolated from adult female fat body. cDNA clones of mRNA for microvitellogenin were isolated by screening the cDNA library with antiserum against microvitellogenin. The results of Northern blot analysis and hybrid selection indicated that the cDNA clone was specific for microvitellogenin. The complete nucleotide sequence of the 834 base pair cDNA insert has been determined by the dideoxy chain termination method. The deduced amino acid sequence was compared with the N-terminal sequence determined by Edman degradation, an amino terminal extension of 17 amino acids appeared to be a signal peptide. The cDNA sequence predicts that the mature microvitellogenin is a protein of 232 amino acids with a calculated molecular weight of 26,201. A comparison of the translated amino acid sequence with the sequences in National Biomedical Research Foundation protein library did not establish any sequence similarity with known proteins. The microvitellogenin gene begins to be expressed in the fat body on the first day of the wandering (prepupal) females as determined by using the cDNA insert as a probe to hybridize with the mRNA for microvitellogenin. The cDNA probe was also used to screen a genomic library of M. sexta, yielding three genomic clones for microvitellogenin. One of them was characterized and it contained the complete microvitellogenin gene. The gene sequence was determined. Comparison to the cDNA sequence showed that the microvitellogenin gene contains an intron near the 5'-end of the non-coding region. The 5'-flanking sequence of the gene has been compared to the same regions of yp genes of Drosophila and vitellogenin genes of locust, some similar sequences have been observed and discussed.


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