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dc.contributor.advisorBriggs, Rober E.en_US
dc.contributor.advisorRamage, Robert T.en_US
dc.contributor.authorClark, Dale Rogers.*
dc.creatorClark, Dale Rogers.en_US
dc.date.accessioned2011-10-31T17:06:01Z
dc.date.available2011-10-31T17:06:01Z
dc.date.issued1988en_US
dc.identifier.urihttp://hdl.handle.net/10150/184354
dc.description.abstractPlants that are heterozygous for genetic markers but do not produce segregating progeny may be suspected of carrying a mutation conditioning apomixis. Seed stocks in which heterozygous plants could be identified phenotypically were treated with a chemical mutagen. These seed stocks were heterozygous for recessive genetic markers, and/or heterozygous for a chromosome translocation. Spikes from heterozygous M1 plants were harvested and seeded in bulk. Spikes from heterozygous M2 plants were harvested and planted in M3 rows. The M3 rows were observed for the absence of segregating progeny and/or were observed cytologically for the presence of a heterozygous translocation. M3 rows not segregating for the genetic markers were crossed onto plants homozygous for the genetic markers. The F1 progenies were observed for an expected ratio of 1 normal: 1 recessive plant. All nonsegregating lines were found to be non-heterozygous. These lines most likely occurred due to seed and pollen contamination or were the result of crossing over between genetic markers. Fertile M2 plants were harvested from the treated heterozygous translocation seed stock. Normally, barley plants heterozygous for a translocation will produce semisterile spikes. Plants that would normally be semisterile but are fertile could be carrying a mutation conditioning apomixis. Progeny of the fertile M2 plants were examined cytologically for the presence of the heterozygous translocation. All selected lines contained the normal seven pairs of chromosomes and were the result of seed or pollen contamination. Seed stocks which could eliminate the problem of contamination in future experiments were developed and discussed. Haploviable mutants closely linked with the male sterile locus, msg2, were isolated in these seed stocks. Haploviable mutants are recognized by upset genetic ratios of alleles linked with the mutant. Selfed progenies of plants carrying a haploviable mutation contained fertile and male sterile plants in about a 1:1 ratio. Mostly male sterile progenies were obtained when plants heterozygous for the haploviable mutant and the male sterile allele were crossed onto male sterile plants. Four lines containing haploviable mutants were evaluated for their usefulness in producing all male sterile lines for hybrid barley production.
dc.language.isoenen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.subjectBarley -- Genetics.en_US
dc.subjectApomixis.en_US
dc.subjectPlant mutation.en_US
dc.titleMethods of screening for induced apomictic mutants in barley (Hordeum vulgare L.).en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.identifier.oclc701108001en_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.leveldoctoralen_US
dc.contributor.committeememberDobrenz, Albert K.en_US
dc.contributor.committeememberVoigt, Robert L.en_US
dc.contributor.committeememberEndrizzi, John E.en_US
dc.identifier.proquest8814224en_US
thesis.degree.disciplinePlant Sciencesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.namePh.D.en_US
refterms.dateFOA2018-08-22T17:40:24Z
html.description.abstractPlants that are heterozygous for genetic markers but do not produce segregating progeny may be suspected of carrying a mutation conditioning apomixis. Seed stocks in which heterozygous plants could be identified phenotypically were treated with a chemical mutagen. These seed stocks were heterozygous for recessive genetic markers, and/or heterozygous for a chromosome translocation. Spikes from heterozygous M1 plants were harvested and seeded in bulk. Spikes from heterozygous M2 plants were harvested and planted in M3 rows. The M3 rows were observed for the absence of segregating progeny and/or were observed cytologically for the presence of a heterozygous translocation. M3 rows not segregating for the genetic markers were crossed onto plants homozygous for the genetic markers. The F1 progenies were observed for an expected ratio of 1 normal: 1 recessive plant. All nonsegregating lines were found to be non-heterozygous. These lines most likely occurred due to seed and pollen contamination or were the result of crossing over between genetic markers. Fertile M2 plants were harvested from the treated heterozygous translocation seed stock. Normally, barley plants heterozygous for a translocation will produce semisterile spikes. Plants that would normally be semisterile but are fertile could be carrying a mutation conditioning apomixis. Progeny of the fertile M2 plants were examined cytologically for the presence of the heterozygous translocation. All selected lines contained the normal seven pairs of chromosomes and were the result of seed or pollen contamination. Seed stocks which could eliminate the problem of contamination in future experiments were developed and discussed. Haploviable mutants closely linked with the male sterile locus, msg2, were isolated in these seed stocks. Haploviable mutants are recognized by upset genetic ratios of alleles linked with the mutant. Selfed progenies of plants carrying a haploviable mutation contained fertile and male sterile plants in about a 1:1 ratio. Mostly male sterile progenies were obtained when plants heterozygous for the haploviable mutant and the male sterile allele were crossed onto male sterile plants. Four lines containing haploviable mutants were evaluated for their usefulness in producing all male sterile lines for hybrid barley production.


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