Fluorescence enhancement and angular scattering by selected cylindrical particles.
AuthorAbromson, David, 1961-
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PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractAn examination of light scattering and emissions from a fluorescent dye confined within small hollow core quartz fibers has revealed several interesting phenomena. First, backscattered fluorescent emissions increased as the fiber's inner diameter decreased below 23 microns. Second, interference between the inner and outer fiber walls created angular scattering intensity oscillations in the elastic scattered light and fluorescent emissions. The interference induced oscillations produced large intensity changes in less than a degree. Third, fiber immersion in an index-matched fluid removed the interference oscillations, and fourth, the fiber immersion also decreased the backscattered fluorescent emissions for the same fiber. These research results can be used to optimize the design of fluorescence measuring devices used in capillary zone electrophoresis (CZE). A CZE device measures the fluorescence spectrum from biological material within small hollow core fibers. The small amount of fluorescent material makes it desirable to find an optimum viewing angle to maximize signal and avoid spurious results from selective wavelength enhancement and intensity variations in angular scattering caused by the fiber.