Design and synthesis of oxytocin agonists based on molecular mechanics and computer modeling.
AdvisorHruby, Victor J.
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PublisherThe University of Arizona.
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AbstractSome space-constraining amino acid-containing oxytocin analogues were synthesized, of which the biological activities were found to be remarkably consistent with the predictions based on molecular mechanics calculations using the CHARMM program. Correlations of the biological activities and computer modeling studies of the conformational properties of Tyr², Phe², eBmp² (agonists), Pen¹, and Tic² (antagonists) oxytocin analogs revealed that a g+ conformation for the aromatic ring in the 2-position is important for the oxytocin-uterus receptor transduction. Examination of the topographical features of the energy minimized conformations of these analogs shows that a parallel aromatic surface over the top of the 20-membered disulfide containing ring of the molecule is equally important for the transduction. Though the Tic compound may exclusively exist as the g+ conformation for the aromatic ring in the 2-position, possible backbone changes and particularly the perpendicularly located aromatic ring on the top of the 20-membered ring may be the reason for its antagonism. Calculations shows that (erythro(2S, 3S)-β-methyltyrosine²) OXT has all the requirements for being an highly active compound, while the isomer (threo-L-(2S, 3R)-β-methyltyrosine²) OXT, which differs only in the configuration of the β carbon, is unlikely to be an agonist according to our calculations. Both compounds were synthesized together with other analogs by the solid-phase peptide synthesis techniques on p-methyl-benzhydrylamine resin. The biological activities of these two compounds were consistent with the predictions.