INFLUENCE OF CYCLOPHOSPHAMIDE ON THE RESPONSE OF MICE TO BOVINE AND PORCINE TRANSFER FACTOR.
AuthorCLARK, PAUL DOUGLAS.
KeywordsTransfer factor (Immunology)
Mice as laboratory animals.
AdvisorWilson, George S.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractCyclophosphamide (CY) was injected intraperitoneally into mice at varying dosage levels 48 hours prior to intravenous inoculation of constant amounts of dialysable leucocytic transfer factor (TF). Bovine TF to coccidioidin and Brucella abortus and porcine TF to Mycobacterium avium were used. Twenty-four hours after TF injections, mice were tested for delayed hypersensitivity by footpad inoculation with specific antigens. The results show that CY slightly enhanced the delayed hypersensitivity response to the specific test antigens in all three systems. This was not true at every CY dose level, however, since there was a correlation between the dosage of CY and the time of peak footpad swelling. Cyclophosphamide at a low level (20 mg/kg body wt) caused the maximal reading at 24 hours after test, whereas the high level dose (200 mg/kg) showed greatest thickening of the footpad at 48 hours. Transfer factor dosage differences appeared to change the response relatively little, if at all. The cyclophosphamide dose response, on the other hand, showed disparities among various antigen systems and at the different observation times. Under these conditions, no consistent, striking effects of CY on transfer could be noted.