• Login
    View Item 
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    The extraction and partial purification of transfer factor from human urine.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    azu_td_9202089_sip1_m.pdf
    Size:
    2.663Mb
    Format:
    PDF
    Description:
    azu_td_9202089_sip1_m.pdf
    Download
    Author
    Wilson, James Leslie.
    Issue Date
    1991
    Keywords
    Dissertations, Academic
    Immunology
    Advisor
    Gerba, Charles
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    The objectives of this study were: (1) to confirm that transfer factor (TF) (also known as dialyzable leukocyte extract) can be extracted from human urine; (2) to develop a more efficient method of extraction, and; (3) to determine the molecular weight of the TF in urine. The study used urine from donors with known skin test reactions to purified protein derivative of Seiberts (PPD-S) and coccidoidin immitis (cocci). For the first study, a forty-eight hour catch of urine and bovine leukocytes were divided into aliquots and processed using dual acetone precipitation followed by dialysis through a 6,000 molecular weight cut off membrane under negative pressure for 4 to 5 days to extract the TF-like substance. Results confirmed that TF activity could be derived from human urine, and that this procedure could be used with blood to extract TF. In the second study, tangential flow filtration combined with stirred cell final filtration and dual acetone precipitation required only 10 hours to produce TF activity nearly identical to the first study. The stirred cell and acetone procedure could also be used to extract TF from leukocyte extracts. Concentrated urine aliquots placed in equilibrium dialysis membranes demonstrated that the most active fraction was the 2,000 dialysate which was consequently subjected to Fast Atom Bombardment (FAB) ionization using a four sector mass spectrometer. This produced two groups of activity: one between 1270-1510, and the other between 1880-2142 daltons. In conclusion, TF can be extracted from human urine. Tangential flow filtration followed by stirred cell filtration can be used to extract the active substance, and the molecular weight of the active substance in urine TF is between 1279-1510 or 1880-2142 daltons.
    Type
    text
    Dissertation-Reproduction (electronic)
    Degree Name
    Ph.D.
    Degree Level
    doctoral
    Degree Program
    Nutritional Sciences
    Graduate College
    Degree Grantor
    University of Arizona
    Collections
    Dissertations

    entitlement

     
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.