• Login
    View Item 
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Molecular biology and evolution of the Rubisco small subunit multi-gene family in Mesembryanthemum crystallinum.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    azu_td_9307652_sip1_m.pdf
    Size:
    4.951Mb
    Format:
    PDF
    Description:
    azu_td_9307652_sip1_m.pdf
    Download
    Author
    De Rocher, Ernest Jay.
    Issue Date
    1992
    Keywords
    Dissertations, Academic.
    Molecular biology.
    Ice plant.
    Committee Chair
    Bohnert, Hans J.
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    The genes that comprise the multi-gene family encoding the small subunit of the CO₂ fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in the facultative Crassulacean Acid Metabolism (CAM) plant Mesembryanthemum crystallinum were isolated and nucleotide sequences were determined. The gene family is comprised of six genes of which four are expressed (rbcS-1, rbcS-2, rbcS-3 and rbcS-4) based on the isolation of corresponding cDNAs and northern analysis. Sequence analysis of the genes reveals influences of gene duplication, gene conversion and selection pressure in the evolution of the gene family. Five of the genes are located in a tandem array within 20 kbp. Two are identical throughout the coding regions and intron sequences. The promoter regions of five of the genes lack regulatory sequence elements conserved in other dicot species. Gene specific probes were used to determine the expression properties of the rbcS-1, rbcS-2, rbcS-3 and rbcS-4 genes during environmental stress, development and the diurnal cycle. The four genes are differentially expressed and are co-regulated in response to stress and during development. Steady state rbcS mRNA amounts are down regulated by NaCl and cold stress. Comparison of changes in transcriptional activities relative to steady state mRNA amounts revealed that NaCl causes increased rbcS RNA turnover. RbcS expression is transcriptionally down regulated coincident with the developmental enablement of CAM induction by stress. The regulation of rbcS expression by changes in RNA turnover is itself developmentally regulated. The rbcS genes of M. crystallinum are transcriptionally regulated during development and are regulated at the level of RNA turnover by stress.
    Type
    text
    Dissertation-Reproduction (electronic)
    Degree Name
    Ph.D.
    Degree Level
    doctoral
    Degree Program
    Molecular and Cellular Biology
    Graduate College
    Degree Grantor
    University of Arizona
    Collections
    Dissertations

    entitlement

     
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.