• Login
    View Item 
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Alleviation of transport limitations in free-flow zone electrophoresis.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    azu_td_9408394_sip1_m.pdf
    Size:
    5.069Mb
    Format:
    PDF
    Description:
    azu_td_9408394_sip1_m.pdf
    Download
    Author
    Sharnez, Rizwan.
    Issue Date
    1993
    Committee Chair
    Sammons, David W.
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    With current free-flow zone electrophoresis (FFZE) technology, particles can be separated if their mobilities differ by more than 5%, at throughputs ranging between 10⁸ and 10⁹ cells/hr. These capabilities are inadequate for most biological applications. The objective of this research was to alleviate the limitations of FFZE through analysis of the underlying transport effects. A mathematical model was developed, which led to a chamber design that amplifies the peak-to-peak distance between sample components δ over five times; a mode of operation that virtually eliminates artifactual dispersion; and a new approach for scale-up. The model predicted the theoretical limit of resolution for symmetrically and asymmetrically cooled chambers to be 0.7% and 4.9% respectively. The latter compares well with published values of around 5%. The significantly higher resolution of symmetrically cooled chambers is explained in terms of the temperature dependence of mobility. The effects of electrosmosis, sedimentation, free convection, and the direction of the flow on resolution were also evaluated. The design feature that amplifies δ in the new chamber is a series of constrictions along the axis of separation. The amplification of δ at each constriction is shown to vary as the cube of the gap-width differential, and is explained in terms of selective increments in residence time and deflection rate of the faster component relative to that of the slower component. In the new mode of operation, called continuous-flow batch electrophoresis, the peaks are two to three times narrower and the distance between them 50% greater as compared to conventional FFZE. The higher resolution is accounted for in terms of uniformity of residence time and the absence of electroosmosis. In the new approach to scale-up, the ability to amplify δ is exploited to reduce the field strength required to obtain a given degree of separation. The disruptive effects of free convection and ohmic heating are thus suppressed by minimizing the heat generated by the field. Based on the findings of this study a new design for enhancing resolution and throughput is proposed. Simulation results indicate that under microgravity with the proposed design resolution would exceed 0.2%, while throughputs would be 10² times greater for cells and 10⁴ times greater for proteins.
    Type
    text
    Dissertation-Reproduction (electronic)
    Degree Name
    Ph.D.
    Degree Level
    doctoral
    Degree Program
    Chemical Engineering
    Graduate College
    Degree Grantor
    University of Arizona
    Collections
    Dissertations

    entitlement

     
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.