BIOCHEMICAL CHARACTERISTICS OF CUCURBITACEAE SALT-SOLUBLE PROTEINS.
AuthorAL-KANHAL, MOHAMAD AHMAD.
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PublisherThe University of Arizona.
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AbstractSalt-soluble proteins of Apodanthera undulata, Cucurbita digitata, C. foetidissima, C. moschata, C. palmeri and C. sororia, were isolated from the decorticated seeds. The isoelectric focusing pattern of A. undulata was divided into two groups, the first falling between pH 4.43 to 5.48 and consisting of six distinct peaks. The pattern of C. digitata consisted of three groups of six, two and three peaks falling in the pH ranges of 5.19 to 6.14, 6.48 to 7.00 and 7.14 to 7.89, respectively. The pattern for C. foetidissima was divided into three groups of four, two and three peaks which fell in the pH ranges 4.82 to 5.82, 6.38 to 6.86 and 7.00 to 8.00, respectively. The patterns of C. palmeri and C. sororia were virtually identical, with two groups of eight and five peaks which fell in the pH range 4.54 to 5.72 and 6.24 to 7.77, respectively. The C. moschata pattern showed three groups of seven, seven and five peaks in the pH range 5.00 to 5.91, 6.00 to 7.00 and 7.24 to 8.29, respectively. On electrophoresis the globulins of all five Cucurbita species displayed six distinct bands with the same relative mobilities, while the globulin pattern of A. undulata was quite different. There was no similarity among the patterns of the Cucurbita albumins, and none resembled that of A. undulata. Two-dimensional analysis of the salt-soluble extracts showed similarity among the five Cucurbita species and differences from A. undulata with respect to the relative mobilities of the resolved bands. Molecular weight determination by gel filtration, resulted in resolution of each globulin fraction into four peaks except that of C. moschata, which was resolved into five peaks. Molecular weight values ranged from 12,000 to 660,000. Each albumin fraction was resolved into three peaks except that of A. undulata which possessed four peaks. The molecular weight values ranged from 11,000 to 446,000.
Degree ProgramAgricultural Biochemistry and Nutrition