AuthorDeshmukh, Ranjit Ravindra.
Committee ChairBier, Milan
Baygents, James C.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractIsotachophoresis (ITP), a mode of electrophoresis, was studied experimentally and theoretically. Although this technique has not been used widely for protein separations, it has features conducive to large scale purification. These include high product concentrations and the use of common biochemical buffers. This technique was applied in this work to purify major proteins of human plasma. Experiments were performed on the Recycling Isotachophoresis (RITP) apparatus, which was developed earlier in our research group. The separations were optimized by varying the buffers, polarity of separation, buffer pH and the use of spacers. Computer simulations were used to optimize these separations. Two novel operating schemes were developed to use the advantages of ITP and scale up the apparatus by semi-continuous operation. The effect of imposed convective flows on the separation process was studied through experiments and computer modeling. Previously developed computer model was modified to simulate convective flows. This work led to identification and use of counterflow (CF) as an additional optimization parameter in ITP. Transport modeling has also been used to study the effect of recycling in the RITP apparatus. The effect of CF velocity profile on ITP steady state zonal structure was analyzed. Imposed CF with parabolic flow profile increases the effective dispersivity in the direction of electromigration, thus reducing the resolution. Uniform plug-flow type counterflows however do not affect the resolution. The improved simulation model incorporating convective flows is also applicable in simulating the effect of electroosmotic flows in analytical capillary electrophoresis.
Degree ProgramChemical Engineering