Author
Bennett, Doyle Edward.Issue Date
1995Committee Chair
O'Brien, David F.
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The University of Arizona.Rights
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.Abstract
The photopolymerization of two-component large unilamellar liposomes (LUV) composed of 3/1 dioleoylphosphatidylethanolamine (DOPE) and either 1,2-bis- (10-(2',4'-hexadienoyloxy)decanoyl) -sn-glycero-3-phosphatidylcholine (bis-SorbPC) or 1-palmitoyl-2- (10-(2$\sp\prime,4\sp\prime$-hexadienoyloxy) decanoyl) -sn-glycero-3-phosphocholine (mono-SorbPC) facilitated liposome fusion. Fusion was characterized by fluorescent assays for lipid mixing, aqueous contents mixing, and aqueous contents leakage. The rate and extent of the photoinduced fusion was dependent on the extent of polymerization, temperature, and the fusion initiation conditions, including the pH and the presence of Mg²⁺ ions. Examination of the temperature dependence of fusion for unpolymerized and polymerized liposomes showed that an enhancement of the rate of fusion occurred in the temperature range, Δ T(I), where inverted phase lipid intermediates are observed. Photopolymerization causes lateral separation of the liposome components and thereby results in the isothermal induction of fusion by lowering the threshold temperature for formation of fusion intermediates.Type
textDissertation-Reproduction (electronic)
Degree Name
Ph.D.Degree Level
doctoralDegree Program
ChemistryGraduate College