LOCALIZATION AND COMPLETE NUCLEOTIDE SEQUENCE OF THE BOVINE PAPILLOMAVIRUS TYPE-2 LATE REGION. POLYPEPTIDE COMPOSITION OF BPV-2.
AuthorPOTTER, HAROLD LEE, JR.
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PublisherThe University of Arizona.
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AbstractThis investigation was designed to partially characterize the genome of bovine papillomavirus type-2 (BPV-2). A closely related virus, bovine papillomavirus type-1 (BPV-1), has been extensively studied. Additionally, many members of the human papillomaviruses (HPV) are being investigated in great detail. However, only a few molecular biology studies of BPV-2 have been reported. Therefore, it seemed appropriate to initiate an investigation whose results would provide a good basis for continued studies of BPV-2. A detailed physical map of BPV-2 DNA was prepared by restriction endonuclease digestion of BPV-2 DNA. A comparison between the genomes of BPV-2 and BPV-1 in regard to location and number of cleavage sites of seven enzymes was performed. This comparison revealed similarities between the two genomes and indicated the probable location of the BPV-2 late region by direct extrapolation from the BPV-1 genomic map. This area was completely sequenced by the chemical method and the results confirmed it to indeed be the BPV-2 late region. Comparative analysis between the BPV-2 late region and the BPV-1 late region demonstrated identical genetic organization to exist along with a high degree of nucleotide sequence conservation. Also, the polypeptide composition of BPV-2 was determined and similarities with these and BPV-1 polypeptides were noted. Lastly, a discussion of the BPV-2 polypeptides as potential products of the BPV-2 late region is presented.
Degree ProgramMicrobiology and Immunology