MORPHO-PHYSIOLOGICAL EVALUATIONS OF ALEPPO AND BRUTIA PINE SEEDLINGS UNDER TWO DIFFERENT MOISTURE REGIMES (SYRIA, AFFORESTATION, CHLOROPLASTS).

Abstract

The mechanism of drought resistance in the seedlings of Aleppo pine (Pinus halepensis) and Brutia pine (Pinus brutia) was investigated. Both species showed anatomical and morphological adaptations to conserve moisture. Aleppo pine had a thicker cuticle, fewer stomata per unit length, per unit area, and per needle than Brutia pine. A significant number of Aleppo pine stomata were sealed with a waxy layer. Brutia pine had shorter needles, smaller needle surface area, a smaller surface area-to-volume ratio, and longer main root length. The two species were similar in height growth and in the seasonal trend of total non-structural carbohydrates (reducing sugars and starch). Brutia pine had more reducing sugars and less starch in its shoots than Aleppo pine. The latter had a greater amount of total non-structural carbohydrates and starch when the seedlings were subjected to dry down moisture stress. Electron microscopy techniques were used to monitor ultrastructural changes in the chloroplasts of mesophyll cells. Aleppo pine was found to contain chloroplasts exhibiting water stress-related damage at a relative water content of 62 percent, where as Brutia pine chloroplasts were disrupted. It is suggested that future investigations examine the physiological manifestation of drought mechanism at the cellular and molecular levels of both species.