• Accumulation of Proline in Germinating Cotton (Gossypium hisutum L.) Seeds During NaCl Stress

      Lehle, F. R.; Zegeer, A. M.; Silvertooth, Jeff; Bantlin, Marguerite (College of Agriculture, University of Arizona (Tucson, AZ), 1990)
      The accumulation of proline (Pro) in plants during NaCl stress may have adaptive significance and the study of this response could reveal a genetic strategy for enhancing NaCl tolerance. Our objective was to determine if the Pro content of germinating cotton seeds is altered by exposure to exogenous NaCl. Seeds were imbibed for 24 h at 32°C before transfer to agar media containing NaCl. After 24 h of NaCl exposure, the Pro contents of radicles and cotyledons were measured using a colorimetric assay. Results showed that Pro contents of both radicles and cotyledons increased as the level of NaCl increased. The bulk of Pro content increases were restricted to the radicle and occurred in the range of NaC1 concentrations from 200 to 400 mM NaCl.
    • Effect of NaCl on the Growth of Germinating Upland Cotton (Gossypium hirsutum L.) Seeds

      Lehle, F. R.; Zegeer, A. M.; Silvertooth, Jeff; Bantlin, Marguerite (College of Agriculture, University of Arizona (Tucson, AZ), 1990)
      Responses to environmental stresses such as excess salinity are difficult to understand if not studied under defined conditions. The objective of this study was to determine if cottonseed radicle and cotyledon growth during germination was affected by exogenous NaCl. Cottonseeds were imbibed in moistened paper toweling for 24 h at 32°C before transfer to an agar media containing selected concentrations of NaCl for an additional 24 h. Radicle linear growth extension was stimulated at low NaC1 concentrations (80 mM), but increasingly inhibited as NaCl concentrations increased from 160 to 400 mM. Radicle and cotyledon fresh weights were increasingly inhibited by NaCl concentrations between 0 and 400 mM. NaCl inhibition of radicle fresh weights was more pronounced than that of cotyledons. We conclude that the inhibitory effects of NaCl can be quantified as reductions in cottonseed radicle and cotyledon growth.
    • Exogenous L- and D-Proline Does Not Reduce NaCl Inhibition of Radidle Growth of Germinating Cotton (Gossypium hirsutum L.) Seeds

      Lehle, F. R.; Zegeer, A. M.; Silvertooth, Jeff; Bantlin, Marguerite (College of Agriculture, University of Arizona (Tucson, AZ), 1990)
      The adaptive significance of proline (Pm) accumulation in cotton seeds can be assessed by determining if NaCl tolerance is influenced by changes in the Pm level of the seed. The objective of this study was to determine if exogenous L-Pro and D-Pro reduce NaCl inhibition of radicle growth of germinating cotton seeds. Seeds were imbibed for 24 h at 32°C before transfer to agar medium containing either Pro, NaCl or mixtures of both. Results indicate that exogenous Pro increases the Pro contents of cotton seed radicles and cotyledons to a similar extent. Such increases are independent of whether L- or D-Pro is fed exogenously. Results also show that exogenous Pro does not significantly reduce NaCl inhibition of radicle growth in germinating cotton seeds. .