Committee ChairDay, A. D.
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PublisherThe University of Arizona.
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AbstractThe Avena Coleoptile Test indicated that there were no indolyl- 3-acetic acid-like growth substances present in treated municipal wastewater from the Activated Sludge Sewage Treatment Plant in Tucson, Arizona. Using the bioassay of kinetin (6-furfurlyamino purine) as a standard in delaying chlorophyll degradation, wastewater extract was capable of maintaining chlorophyll in wheat (Triticum aestivum L.) leaf tissue; however, toxic contaminants present in municipal wastewater may have prevented a consistent dose response. In the process of repeated chromatography, kinetin broke down into two zones that were capable of retarding chlorophyll degradation. After repeated chromatography, wastewater extract and degradation products of kinetin resulted in similar Rf values. Wastewater extract obtained through repeated chromatography resulted in increased total chlorophyll retention, with increased concentrations; however, the nature of the cytokinin-like growth substances present in wastewater appeared to be unique. The high yields of high protein plant products obtained from plants grown with wastewater may have resulted from cytokinin-like growth substances present in treated municipal wastewater.
Degree ProgramPlant Sciences