Comparative Transport of Bacteriophage and Microspheres in an Aquifer Under Forced-Gradient Conditions.
AuthorLenczewski. Melissa E.
Watershed management -- Arizona.
Committee ChairGerba, C. P.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractThe transport of viruses in groundwater was studied by using two bacteriophages (PRD-1 and M-1) and carboxylated latex microspheres (100 nm) under forced gradient conditions. This study was also designed to determine the effects of a change in pH of the aquifer on the transport of viruses and microspheres, and if the microspheres reflected the transport of viruses. The microspheres, bacteriophage, and chloride tracers were injected for a period of 12 hours into a fine- to medium-sand aquifer, 9 to 10 m in depth underlayed by a clay silt bed. Samples were collected 1, 2.5, 4, and 6 m distant from the injection well. After 10 days the pH of the groundwater was increased from 7.5 to 8.5 and samples collected for an additional 7 days. The results of this study indicated that a small increase in the pH of an aquifer can cause detachment of viruses and microspheres from the soil. In addition, the transport behavior of the bacteriophage was found to be different from the carboxylated microspheres.
Degree ProgramSoil and Water Sciences