Development of a microporous filter method for concentration of rotavirus from tap water
Committee ChairGerba, Charles P.
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PublisherThe University of Arizona.
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AbstractAn adsorption-elution method was developed for the concentration of the simian rotavirus SAll from tap water. Negatively and positively charged microporous filters were compared for their suitability for rotamirus adsorption efficiency from water and sewage. The positively charged filters were far superior to the negatively charged filters in their adsorption efficiency at ambient pH. Both, the Zeta-Plus (50S) and the Virozorb 1 MDS were equally efficient in their adsorption capacities, but the elution efficiency of the 1 MDS exceeded that of the Zeta-Plus. Tryptose phosphate broth was found to be the best eluent for rotavirus. For small volumes of seeded tap water the percent recovery of rotavirus was 45.5 ± 0.7% for Zeta-Plus and 78.5 ± 3.5% for 1 MDS. However, with large volumes of input water, the percent recoveries were reduced to 13.7 ± 0.57% for Zeta-Plus and 19.5 ± 0.7% for 1 MDS.