Characterization of Membrane Permeability and Polymer-Stabilized Model Membranes
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Author
Ma, YaningIssue Date
2007Keywords
Giant unilamellar vesicleEpi-fluorescence
Polymer
Mass spectrometry
Gel permeation chromatography
Advisor
Saavedra, Steve ScottCommittee Chair
Saavedra, Steve Scott
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The University of Arizona.Rights
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.Abstract
The permeability of lipid bilayer membranes to glucose and carboxyfluorescein has been studied in model membranes. Using an enzyme assay, the permeability of glucose was monitored spectrometrically with both large and giant unilamellar vesicles (LUVs and GUVs). The permeability of carboxyfluorescein was studied by entrapping the dye and monitoring its leakage over time from a single GUV. Permeability study using GUVs may provide new information that cannot be obtained from LUVs.The stability of lipid membranes was enhanced by incorporating polymer scaffold. LUVs were prepared with hydrophobic monomers partitioned and then polymerized inside the hydrophobic interior of the lipid bilayers. The sizes of the formed polymers were characterized using gel permeation chromatography and mass spectrometry. This study suggests that large molecular weight polymers were formed inside the lipid bilayers and that the stability of the membranes is related to the size of the polymers.Type
textElectronic Thesis
Degree Name
MSDegree Level
mastersDegree Program
ChemistryGraduate College