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    Characterization of Membrane Permeability and Polymer-Stabilized Model Membranes

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    Author
    Ma, Yaning
    Issue Date
    2007
    Keywords
    Giant unilamellar vesicle
    Epi-fluorescence
    Polymer
    Mass spectrometry
    Gel permeation chromatography
    Advisor
    Saavedra, Steve Scott
    Committee Chair
    Saavedra, Steve Scott
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    The permeability of lipid bilayer membranes to glucose and carboxyfluorescein has been studied in model membranes. Using an enzyme assay, the permeability of glucose was monitored spectrometrically with both large and giant unilamellar vesicles (LUVs and GUVs). The permeability of carboxyfluorescein was studied by entrapping the dye and monitoring its leakage over time from a single GUV. Permeability study using GUVs may provide new information that cannot be obtained from LUVs.The stability of lipid membranes was enhanced by incorporating polymer scaffold. LUVs were prepared with hydrophobic monomers partitioned and then polymerized inside the hydrophobic interior of the lipid bilayers. The sizes of the formed polymers were characterized using gel permeation chromatography and mass spectrometry. This study suggests that large molecular weight polymers were formed inside the lipid bilayers and that the stability of the membranes is related to the size of the polymers.
    Type
    text
    Electronic Thesis
    Degree Name
    MS
    Degree Level
    masters
    Degree Program
    Chemistry
    Graduate College
    Degree Grantor
    University of Arizona
    Collections
    Master's Theses

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