AuthorHu, Fangle (Carol)
AdvisorZinsmaier, Konrad E.
Committee ChairZinsmaier, Konrad E.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractThe Miro protein, which contains two GTPase domains flanking two EF-hand motifs, may be a candidate to link intracellular signaling with mitochondrial transport. We found that increasing Drosophila Miro (dMiro) activity by overexpression almost abolishes the mitochondrial distribution in the neuropil ventral ganglia but not in axons of nerves. Studies of a potential temperature-sensitive (ts) mutation in the 2nd GTPase domain (G456E) showed insignificant difference in mitochondrial distribution in comparison to motor neurons overexpressing wild type dMiro at 25°C. There is also no dramatic difference between G456E expression at 18°C and 27°C, suggesting that the mutation may not act as a dominant-negative ts-mutation. Analysis mutant dMiro protein that is deficient for both EF-hand motifs (E234K/E321K) shows a wild type-like subcellular distribution of mitochondria much in contrast to the dominant effects of overexpressing normal dMiro. This suggests that both EF hands are required for the dominant effects of normal Miro overexpression.
Degree ProgramMolecular & Cellular Biology