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dc.contributor.advisorZinsmaier, Konrad E.en_US
dc.contributor.authorHu, Fangle (Carol)
dc.creatorHu, Fangle (Carol)en_US
dc.date.accessioned2011-12-05T14:14:55Z
dc.date.available2011-12-05T14:14:55Z
dc.date.issued2007en_US
dc.identifier.urihttp://hdl.handle.net/10150/193358
dc.description.abstractThe Miro protein, which contains two GTPase domains flanking two EF-hand motifs, may be a candidate to link intracellular signaling with mitochondrial transport. We found that increasing Drosophila Miro (dMiro) activity by overexpression almost abolishes the mitochondrial distribution in the neuropil ventral ganglia but not in axons of nerves. Studies of a potential temperature-sensitive (ts) mutation in the 2nd GTPase domain (G456E) showed insignificant difference in mitochondrial distribution in comparison to motor neurons overexpressing wild type dMiro at 25°C. There is also no dramatic difference between G456E expression at 18°C and 27°C, suggesting that the mutation may not act as a dominant-negative ts-mutation. Analysis mutant dMiro protein that is deficient for both EF-hand motifs (E234K/E321K) shows a wild type-like subcellular distribution of mitochondria much in contrast to the dominant effects of overexpressing normal dMiro. This suggests that both EF hands are required for the dominant effects of normal Miro overexpression.
dc.language.isoENen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.titleAnalysis of dMiro's Protein Domains in Drosophilaen_US
dc.typetexten_US
dc.typeElectronic Thesisen_US
dc.contributor.chairZinsmaier, Konrad E.en_US
dc.identifier.oclc659748163en_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.levelmastersen_US
dc.contributor.committeememberZinsmaier, Konrad E.en_US
dc.contributor.committeememberZarnescu, Daniela C.en_US
dc.contributor.committeememberNighorn, Alanen_US
dc.identifier.proquest2308en_US
thesis.degree.disciplineMolecular & Cellular Biologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.nameMSen_US
refterms.dateFOA2018-06-13T00:51:04Z
html.description.abstractThe Miro protein, which contains two GTPase domains flanking two EF-hand motifs, may be a candidate to link intracellular signaling with mitochondrial transport. We found that increasing Drosophila Miro (dMiro) activity by overexpression almost abolishes the mitochondrial distribution in the neuropil ventral ganglia but not in axons of nerves. Studies of a potential temperature-sensitive (ts) mutation in the 2nd GTPase domain (G456E) showed insignificant difference in mitochondrial distribution in comparison to motor neurons overexpressing wild type dMiro at 25°C. There is also no dramatic difference between G456E expression at 18°C and 27°C, suggesting that the mutation may not act as a dominant-negative ts-mutation. Analysis mutant dMiro protein that is deficient for both EF-hand motifs (E234K/E321K) shows a wild type-like subcellular distribution of mitochondria much in contrast to the dominant effects of overexpressing normal dMiro. This suggests that both EF hands are required for the dominant effects of normal Miro overexpression.


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