Development and Validation of a Novel Assay to Quantify D-Limonene in Human Adipose Biopsies
AuthorMiller, Jessica Anne
Committee ChairThomson, Cynthia
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstractd-Limonene is a lipid-soluble bioactive food component found in citrus peel. It has demonstrated strong chemopreventive effects in rodent mammary, gastric, skin, liver, and lung cancers and is correlated with a significantly reduced risk for human squamous cell skin carcinoma. Because d-limonene is fat-soluble, it may have enhanced chemopreventive activity in fatty tissues such as breast. No previous methodology to quantify d-limonene in the adipose had been developed which significantly limited d-limonene tissue distribution research. For this research, an assay to extract d-limonene from adipose tissue and quantify with GC/MS was developed and validated. Linear calibration curves were established over the range of 0.0-2,526 ng d-limonene. Extraction recovery was 80%. Satisfactory within day precision (RSD 6.75 to 9.56%) and accuracy (% difference of 2-4%) were achieved. This sensitive, accurate, and precise assay to quantify d-limonene in adipose can be used for future human or animal fatty tissue deposition studies.
Degree ProgramNutritional Sciences