Formation of Dicentric and Acentric Chromosomes, by a Template Switch Mechanism, in Budding Yeast
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Author
Paek, Andrew LutherIssue Date
2010Keywords
Acentric ChromosomesBudding Yeast
Dicentric Chromosomes
Genome Instability
Inverted Repeats
Template Switching
Advisor
Weinert, TedCommittee Chair
Weinert, Ted
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The University of Arizona.Rights
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.Abstract
Chromosomal rearrangements occur in all organisms and are important both in the evolution of species and in pathology. In this dissertation I show that in Saccharomyces cerevisiae, or budding yeast, one type of chromosomal rearrangement occurs when inverted repeats fuse, likely during DNA replication by a novel mechanism termed "faulty template switching". This fusion can lead to the formation of either a dicentric or acentric chromosome, depending on the direction of the replication fork. Dicentric chromosomes are inherently unstable due to their abnormal number of centromeres, and thus undergo additional chromosomal rearrangements and chromosome loss.Type
textElectronic Dissertation
Degree Name
Ph.D.Degree Level
doctoralDegree Program
Molecular & Cellular BiologyGraduate College