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dc.contributor.authorRodriguez, Roberto Alejandro*
dc.creatorRodriguez, Roberto Alejandroen_US
dc.date.accessioned2011-12-05T22:36:02Z
dc.date.available2011-12-05T22:36:02Z
dc.date.issued2007en_US
dc.identifier.urihttp://hdl.handle.net/10150/194483
dc.description.abstractThe goals of this dissertation were to determine the viral contribution of combined sewer overflows (CSO) to receiving waters during wet weather conditions, and to compare the use of the primary liver carcinoma (PLC/PRF/5) cells with the buffalo green monkey (BGM) cells for total culturable virus assay (TCV). To assess the contribution of CSO on the viral quality of the receiving water, samples of discharges and effluent receiving waters of three sewage reclamation plants located in Illinois were collected from June to October during two consecutive years. Samples were tested for TCV, adenovirus and norovirus. Viral concentration in the receiving water increases approximately ten times the concentration during CSO events in comparison with dry weather. An assessment comparing TCV by PLC/PRF/5 and BGM cells was also conducted using sewage samples collected before and after disinfection. PLC/PRF/5 cells detected between 10 to 50 times more viruses (?) than the BGM cells. Adenoviruses were detected in the PLC/PRF/5 cells, but not in the BGM cells. In conclusion, CSO events resulted in a significant increase in the concentration of viruses in the receiving waters and PLC/PRF/5 cells are more sensitive for enteric virus detection than the BGM cell line.
dc.language.isoENen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.titleOccurence of Enteric Viruses on Combined Sewer Overflowsen_US
dc.typetexten_US
dc.typeElectronic Dissertationen_US
dc.contributor.chairGerba, Charles P.en_US
dc.identifier.oclc659748081en_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.leveldoctoralen_US
dc.contributor.committeememberGerba, Charles P.en_US
dc.contributor.committeememberMaier, Rainaen_US
dc.contributor.committeememberReynolds, Kellyen_US
dc.contributor.committeememberPepper, Ianen_US
dc.identifier.proquest2236en_US
thesis.degree.disciplineSoil, Water & Environmental Scienceen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.namePhDen_US
refterms.dateFOA2018-06-25T01:28:11Z
html.description.abstractThe goals of this dissertation were to determine the viral contribution of combined sewer overflows (CSO) to receiving waters during wet weather conditions, and to compare the use of the primary liver carcinoma (PLC/PRF/5) cells with the buffalo green monkey (BGM) cells for total culturable virus assay (TCV). To assess the contribution of CSO on the viral quality of the receiving water, samples of discharges and effluent receiving waters of three sewage reclamation plants located in Illinois were collected from June to October during two consecutive years. Samples were tested for TCV, adenovirus and norovirus. Viral concentration in the receiving water increases approximately ten times the concentration during CSO events in comparison with dry weather. An assessment comparing TCV by PLC/PRF/5 and BGM cells was also conducted using sewage samples collected before and after disinfection. PLC/PRF/5 cells detected between 10 to 50 times more viruses (?) than the BGM cells. Adenoviruses were detected in the PLC/PRF/5 cells, but not in the BGM cells. In conclusion, CSO events resulted in a significant increase in the concentration of viruses in the receiving waters and PLC/PRF/5 cells are more sensitive for enteric virus detection than the BGM cell line.


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