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dc.contributor.advisorDescour, Michael Ren_US
dc.contributor.authorRogers, Jeremy Daviden_US
dc.creatorRogers, Jeremy Daviden_US
dc.date.accessioned2011-12-05T22:36:17Z
dc.date.available2011-12-05T22:36:17Z
dc.date.issued2006en_US
dc.identifier.urihttp://hdl.handle.net/10150/194489
dc.description.abstractSuccessful treatment of many types of cancer is improved when early detection is possible. One method of early detection is microscopic inspection of the tissue at risk. Microscopic inspection can be performed by extracting a biopsy and using traditional microscopes, but biopsy is painful and inconvenient which limits its use. An alternative is in vivo microscopy using an endoscope or microendoscopy. This dissertation describes the design, construction, and evaluation of a miniature microscope or microendoscope based on new microfabrication technologies.All components typically found in a traditional bench-top microscope were designed and built on a miniature scale. The objective was comprised of one planoconvex spherical glass lens and three printed microlenses. The printed lenses were patterned using grayscale lithography in a custom engineered photosensitive hybrid sol-gel glass material. Illumination was delivered by high-brightness Light Emitting Diodes (LEDs) via multimode fiber. The design incorporated a custom imaging detector and a Micro-Electrical-Mechanical-Systems (MEMS) actuator for optical sectioning using structured illumination. The opto-mechanical system is designed using a new concept called "zero alignment assembly" in which the lens elements snap into place and are constrained to a precise position with tolerances tighter than the optical tolerances. This scheme requires no post-assembly alignment or adjustment and simplifies system assembly. The miniature microscope was designed to image in several modes including reflectance, fluorescence, and using structured illumination for optical sectioning. A unique optical design incorporated tilted elements to remove ghost images and internal reflections from the image plane. This design enabled microscopic imaging of extremely low reflectance samples like tissue where the "in-focus" component of the object reflects only 0.04% of the illumination. The miniature microscope was built and tested by imaging a variety of test objects including cancer tissue phantoms and pig tissue. The results demonstrated the successful implementation of many new microfabrication technologies and design concepts to build a working prototype miniature microscope measuring only 3x4x15 mm capable of imaging cellular structure of tissue in reflectance or flourescence.
dc.language.isoENen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.titleMiniature Microscope Design And Construction Based On Tilted Rotationally Asymmetric Printed Lensesen_US
dc.typetexten_US
dc.typeElectronic Dissertationen_US
dc.contributor.chairDescour, Michael Ren_US
dc.identifier.oclc659746536en_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.leveldoctoralen_US
dc.contributor.committeememberGmitro, Arten_US
dc.contributor.committeememberShack, Rolanden_US
dc.identifier.proquest1960en_US
thesis.degree.disciplineOptical Sciencesen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.namePhDen_US
refterms.dateFOA2018-08-25T01:08:06Z
html.description.abstractSuccessful treatment of many types of cancer is improved when early detection is possible. One method of early detection is microscopic inspection of the tissue at risk. Microscopic inspection can be performed by extracting a biopsy and using traditional microscopes, but biopsy is painful and inconvenient which limits its use. An alternative is in vivo microscopy using an endoscope or microendoscopy. This dissertation describes the design, construction, and evaluation of a miniature microscope or microendoscope based on new microfabrication technologies.All components typically found in a traditional bench-top microscope were designed and built on a miniature scale. The objective was comprised of one planoconvex spherical glass lens and three printed microlenses. The printed lenses were patterned using grayscale lithography in a custom engineered photosensitive hybrid sol-gel glass material. Illumination was delivered by high-brightness Light Emitting Diodes (LEDs) via multimode fiber. The design incorporated a custom imaging detector and a Micro-Electrical-Mechanical-Systems (MEMS) actuator for optical sectioning using structured illumination. The opto-mechanical system is designed using a new concept called "zero alignment assembly" in which the lens elements snap into place and are constrained to a precise position with tolerances tighter than the optical tolerances. This scheme requires no post-assembly alignment or adjustment and simplifies system assembly. The miniature microscope was designed to image in several modes including reflectance, fluorescence, and using structured illumination for optical sectioning. A unique optical design incorporated tilted elements to remove ghost images and internal reflections from the image plane. This design enabled microscopic imaging of extremely low reflectance samples like tissue where the "in-focus" component of the object reflects only 0.04% of the illumination. The miniature microscope was built and tested by imaging a variety of test objects including cancer tissue phantoms and pig tissue. The results demonstrated the successful implementation of many new microfabrication technologies and design concepts to build a working prototype miniature microscope measuring only 3x4x15 mm capable of imaging cellular structure of tissue in reflectance or flourescence.


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