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dc.contributor.advisorWilliams, Stuart K.en_US
dc.contributor.authorSmith, Cynthia Miller
dc.creatorSmith, Cynthia Milleren_US
dc.date.accessioned2011-12-06T13:23:30Z
dc.date.available2011-12-06T13:23:30Z
dc.date.issued2005en_US
dc.identifier.urihttp://hdl.handle.net/10150/194778
dc.description.abstractTissue loss and end-stage organ failure caused by disease or injury are two of the most costly problems encountered in modern medicine. To combat these problems, a relatively new field, called tissue engineering, has emerged. This field combines the medical and engineering fields in hopes of establishing an effective method to restore, maintain, or improve damaged tissue. In order to best replace the diseased tissue, many approaches to fabricating new tissue have focused on trying to replicate native tissue. The overall hypothesis of this dissertation is that a direct-write, BioAssembly Tool (BAT) can be utilized to fabricate viable constructs of cells and matrix that have a specified spatial organization and are truly three-dimensional (3D). The results of the studies within this dissertation demonstrate that the BAT can generate viable, spatially organized constructs comprised of cells and matrix by carefully controlling the environmental parameters of the system. A joint hypothesis associated with this dissertation is that 3D microscopy and image processing techniques can be combined to generate accurate representative stacks of images of the tissue within 3D, tissue engineered constructs. The results of the studies examining this hypothesis demonstrate that by taking into account the attenuation with depth in the imaged construct as well as by looking at the intensity and gradient of each voxel, accurate and reproducible thresholding can be achieved. Furthermore, this tool can be utilized to aid in the characterization of 3D tissue engineered constructs. Based on these studies, 3D microscopy and image processing shows promise in accurately representing the cellular volume within a tissue. More importantly, 3D, direct-write technology, specifically the BioAssembly Tool, could be used in the fabrication of viable, spatially organized constructs that can then be implanted into a patient to provide healthy tissue in the place of diseased or damaged tissue.
dc.language.isoENen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.subjecttissue engineeringen_US
dc.subjectrapid prototypingen_US
dc.subjectregenerative medicineen_US
dc.titleA Direct-Write Three-Dimensional Bioassembly Tool for Regenerative Medicineen_US
dc.typetexten_US
dc.typeElectronic Dissertationen_US
dc.contributor.chairWilliams, Stuart K.en_US
dc.identifier.oclc137355085en_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.leveldoctoralen_US
dc.contributor.committeememberBarton, Jennifer K.en_US
dc.contributor.committeememberHoying, James B.en_US
dc.contributor.committeememberLynch, Ronald M.en_US
dc.contributor.committeememberWarren, William L.en_US
dc.identifier.proquest1335en_US
thesis.degree.disciplineBiomedical Engineeringen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.namePhDen_US
refterms.dateFOA2018-07-01T15:55:43Z
html.description.abstractTissue loss and end-stage organ failure caused by disease or injury are two of the most costly problems encountered in modern medicine. To combat these problems, a relatively new field, called tissue engineering, has emerged. This field combines the medical and engineering fields in hopes of establishing an effective method to restore, maintain, or improve damaged tissue. In order to best replace the diseased tissue, many approaches to fabricating new tissue have focused on trying to replicate native tissue. The overall hypothesis of this dissertation is that a direct-write, BioAssembly Tool (BAT) can be utilized to fabricate viable constructs of cells and matrix that have a specified spatial organization and are truly three-dimensional (3D). The results of the studies within this dissertation demonstrate that the BAT can generate viable, spatially organized constructs comprised of cells and matrix by carefully controlling the environmental parameters of the system. A joint hypothesis associated with this dissertation is that 3D microscopy and image processing techniques can be combined to generate accurate representative stacks of images of the tissue within 3D, tissue engineered constructs. The results of the studies examining this hypothesis demonstrate that by taking into account the attenuation with depth in the imaged construct as well as by looking at the intensity and gradient of each voxel, accurate and reproducible thresholding can be achieved. Furthermore, this tool can be utilized to aid in the characterization of 3D tissue engineered constructs. Based on these studies, 3D microscopy and image processing shows promise in accurately representing the cellular volume within a tissue. More importantly, 3D, direct-write technology, specifically the BioAssembly Tool, could be used in the fabrication of viable, spatially organized constructs that can then be implanted into a patient to provide healthy tissue in the place of diseased or damaged tissue.


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