• Login
    View Item 
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    An Examination of the Effects and Possible Targets of Nitric Oxide on Olfactory Neurons in the Moth, Manduca Sexta

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    azu_etd_1431_sip1_m.pdf
    Size:
    24.07Mb
    Format:
    PDF
    Description:
    azu_etd_1431_sip1_m.pdf
    Download
    Author
    Wilson, Caroline Hamilton
    Issue Date
    2005
    Keywords
    olfaction
    Manduca sexta
    moth
    nitric oxide
    gaseous neurotransmitters
    Advisor
    Nighorn, Alan J
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    The gaseous messenger, nitric oxide (NO), has emerged as a key component of olfactory systems. Localization and imaging studies in the moth, Manduca sexta, suggest that NO may affect the excitability of olfactory neurons by modifying neuronal membrane properties through sGC-dependent mechanisms. This hypothesis was tested using a multidisciplinary approach, including two types of physiological recording techniques and immunocytochemical analysis of sGC antibody expression in the Manduca brain. The excitability of large populations or individual antennal lobe (AL) neurons was monitored with in vivo physiological recordings while various NO pharmacological agents were bath applied to the brain. To examine possible targets of NO, the binding site of sGC was blocked and the results were compared to NO blockade. Finally, sGC immunocytochemistry was used to also determine possible targets of NO.Two NO synthesis inhibitors and a sGC blocker were potent effectors of resting, baseline activity in the Manduca brain. Blocking NO synthesis caused significant decreases in AL neuron conductance. This conductance decrease led to changes in baseline activity, including the appearance of bursts in some neurons, and increased and decreased firing rates in other neurons. Further, the neurons had a decreased responsiveness and excitability to presynaptic input. Blocking the sGC binding site caused similar effects in most neurons, which indicates that NO likely acts through sGC-dependent signaling to exert its effects in at least a subset of neurons. However, some neurons had different responses to NO and sGC blockade, which indicates that NO may act through other signaling mechanisms in some neurons. Further examination using sGC immunocytochemistry revealed that only about 90% of projection neurons (PNs) and 30% of local neurons (LNs) contained sGC immunoreactivity.The results in this dissertation indicate that NO performs a global function in the antennal lobe to maintain the resting membrane conductance of AL neurons. NO likely exerts its effects through both sGC-dependent and sGC-independent mechanisms. Finally, these results have major implications for odor coding in all species, as NO has been found in the olfactory systems of every animal examined thus far.
    Type
    text
    Electronic Dissertation
    Degree Name
    PhD
    Degree Level
    doctoral
    Degree Program
    Neuroscience
    Graduate College
    Degree Grantor
    University of Arizona
    Collections
    Dissertations

    entitlement

     
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.