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dc.contributor.authorTaylor, Kathryn
dc.contributor.authorEllis, Danielle
dc.contributor.editorWright, Glennen_US
dc.date.accessioned2012-04-25T18:04:06Z
dc.date.available2012-04-25T18:04:06Z
dc.date.issued1996-09
dc.identifier.urihttp://hdl.handle.net/10150/220516
dc.description.abstractPrevious literature concerning citrus and other tree crops led us to ask if there was molecular evidence for mycoplasma -like organisms (MLOs) as the causal agent of Macrophylla decline and two other decline diseases, citrus blight and lemon sieve tube necrosis. We had molecular probes available to us that were either specific for MLOs of tree diseases and others that were universal for all known types of MLOs. We used a polymerase chain reaction (MLO) to determine if MLOs were present in the vascular tissues of decline and healthy citrus. I all trials performed, the trees were negative for MLO-PCR products. In addition, we attempted to transmit putative MLO 's from decline affected trees to Vinca rosea MLO-nurse plants. We were unable to affect this type of transfer. In addition, our attempts to identify MLO's in phloem tissue gave us negative results. We have since revised our hypothesis. We are currently pursuing the hypothesis that these decline disorders are the result of a rootstock scion incompatibility, that we may be able to avoid culturally, while maintaining these valuable combinations.
dc.description.sponsorshipCitrus Research Councilen_US
dc.language.isoen_USen_US
dc.publisherCollege of Agriculture, University of Arizona (Tucson, AZ)en_US
dc.relation.ispartofseriesSeries P-105en_US
dc.relation.ispartofseries370105en_US
dc.subjectAgriculture -- Arizonaen_US
dc.subjectCitrus fruits -- Arizonaen_US
dc.subjectCitrus fruits -- Diseasesen_US
dc.titleMycoplasma-like Organisms as the Causal Agent for Macrophylla Declineen_US
dc.typetexten_US
dc.typeArticleen_US
dc.identifier.journalCitrus Research Reporten_US
refterms.dateFOA2018-08-26T12:33:13Z
html.description.abstractPrevious literature concerning citrus and other tree crops led us to ask if there was molecular evidence for mycoplasma -like organisms (MLOs) as the causal agent of Macrophylla decline and two other decline diseases, citrus blight and lemon sieve tube necrosis. We had molecular probes available to us that were either specific for MLOs of tree diseases and others that were universal for all known types of MLOs. We used a polymerase chain reaction (MLO) to determine if MLOs were present in the vascular tissues of decline and healthy citrus. I all trials performed, the trees were negative for MLO-PCR products. In addition, we attempted to transmit putative MLO 's from decline affected trees to Vinca rosea MLO-nurse plants. We were unable to affect this type of transfer. In addition, our attempts to identify MLO's in phloem tissue gave us negative results. We have since revised our hypothesis. We are currently pursuing the hypothesis that these decline disorders are the result of a rootstock scion incompatibility, that we may be able to avoid culturally, while maintaining these valuable combinations.


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