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    Assessment of the Survival of Microbial Pathogens in the Environment

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    Author
    Williams, David Lee
    Issue Date
    2012
    Keywords
    Microbiology
    Advisor
    Gerba, Charles P.
    
    Metadata
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    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    The studies presented here evaluate the survival of different types of pathogens in a variety of environments. The study Appendix A focuses on the presence of enteric bacteria in reusable shopping bags. We demonstrated that ninety-seven percent of individuals surveyed never washed their reusable shopping bags and that this lack of washing can lead to the buildup of potentially harmful bacteria such as Salmonella spp. The hand-washing of the bags was shown to reduce seeded organisms to below detectable limits. Appendix B examines the survival of Bacillus altrophaeus endospores during household laundering. It was demonstrated that detergent alone didn't significantly reduce the number of viable endospores or their spread to other garments and that bleach is necessary to significantly reduce the number of viable endospores and their spread. Risks for infection were significantly lower when bleach was used during laundering. Appendix C details the survival of Ascaris ova in biosolid-amended Brazito sandy loam and clay loam. Survival of Ascaris ova was significantly higher in clay soil and ova inactivation increased with increasing temperature. The risk for Ascaris infection from consuming raw lettuce grown on such soils was calculated and it was found that annual risks for infection decreased significantly with time after harvest.
    Type
    text
    Electronic Dissertation
    Degree Name
    Ph.D.
    Degree Level
    doctoral
    Degree Program
    Graduate College
    Microbiology
    Degree Grantor
    University of Arizona
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