Characterization of a Gene Targeted by Mitochondrial Retrograde Regulaiton in Arabidopsis Thaliana
PublisherThe University of Arizona.
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AbstractWhen under environmental stresses such as pathogen attack, plant mitochondria initiate a signaling pathway, called mitochondrial retrograde regulation (MRR), to alter nuclear gene expression in order to help the plant survive the stress (4). The Rhoads Lab identified an Arabidopsis thaliana (Arabidopsis) gene (AtSg40690, which may encode an A TP synthase assembly protein) that is strongly increased in expression by MRR and during plant stresses. This project was focused on the development of constitutive expressor (CE) lines of AtSg40690. Only one CE line was generated due to low transformation rates, which might indicate lethality. It is possible that transformants might induce a change in another protein, causing variegation in plants. Using classical molecular biology and microbial genetics techniques, the research group plans to verify the expression patterns in the CE lines and knock out (KO) lines of AtSg40690 and test KO lines and other A TP synthase assembly protein (AAP) gene over-expressor lines to determine if these genes play a role in MRR and in stress responses in plants.
Degree ProgramHonors College
Molecular and Cellular Biology