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    LIPOXYGENASE ACTIVITY ASSOCIATED WITH CYANIDE-INSENSITIVE OXYGEN UPTAKE IN MITOCHONDRIAL FRACTIONS FROM SEEDLINGS OF GLYCINE MAX L

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    Author
    Scherban, Donna Michele, 1954-
    Issue Date
    1987
    Keywords
    Lipoxygenases.
    Soybean -- Seedlings.
    Plant mitochondria.
    
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    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    Soybean seeds are known to contain high levels of lipoxygenase activity, especially during early stages of germination. Crude mitochondrial fractions from germinating soybeans also have been shown to exhibit high rates of cyanide-insensitive oxygen uptake. These results show the effects of successive discontinuous PercollR density gradients on mitochondrial fractions from 2 day old soybean seeds as judged by polarographic studies and Ouchterlony double diffusion. Axis mitochondria exhibited totally cyanide-sensitive oxygen uptake after two gradients and cotyledon mitochondria exhibited from none to 11% cyanide-insensitive oxygen uptake after three gradients. Mitochondrial fractions which were assayed for lipoxygenase with double diffusion exhibited positive results with fractions that showed cyanide-insensitive oxygen uptake and negative results with cyanide-sensitive mitochondria. These results suggest that lipoxygenase can loosely associate with the mitochondrial membrane and that gradient centrifugation can purify mitochondria free of both lipoxygenase and cyanide-insensitive oxygen uptake.
    Type
    text
    Thesis-Reproduction (electronic)
    Degree Name
    M.S.
    Degree Level
    masters
    Degree Program
    Graduate College
    Plant Sciences
    Degree Grantor
    University of Arizona
    Collections
    Master's Theses

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