Response of Corynebacterium pseudotuberculosis to cultivation in media of differing composition
AuthorYeh, Shi-Lian, 1959-
AdvisorSonger, Glenn J.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractCorynebacterium pseudotuberculosis is a pathogenic bacterium which cause suppurative infection mainly in sheep, goats, horses, and other species. It plays a major role in the economy of sheep production worldwide. It produces a toxic phospholipase D which may be involved in pathogenesis. The relationship of physiologic differences between the two biovars of the organism to rates of growth and toxin production are not known. In this work, isolates of C.pseudotuberculosis were examined for relative ability to grow in brain heart infusion broth and a semidefined medium. The effects of carbohydrate source and the presence or absence of various ions were also examined. Results revealed no statistically significant differences between isolates or biovars in any characteristic studied, including growth rate, pH change during growth and PLD production. The semidefined medium provides a reasonable alternative to more complex media for further work in purification of PLD and study of its structure and function.
Degree ProgramGraduate College
Microbiology and Immunology