Genotypic variation in susceptibility of Pisum sativum to crown gall and characterization of one cultivar of pea with reduced susceptibility to crown gall
AuthorRobbs, Steven Lynn, 1961-
AdvisorHawes, Martha C.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractThirty-four cultivars of pea (Pisum sativum) were assayed for tumorigenesis after inoculation with Agrobacterium tumefaciens strain B6. The response of the 34 cultivars fell into 3 significantly different groups based on tumor weights: high, medium, and low susceptibility. The least susceptible cultivar, Sweet Snap, also formed the smallest tumors in comparison to 5 other cultivars when inoculated with 5 other strains of Agrobacterium. Preliminary experiments indicate that neither chemotaxis, binding, vir-gene induction, nor T-DNA expression are limiting factors in Sweet Snap's reduced susceptibility. In an inheritance study, the F1, F2, and F3 progeny from an initial cross between Sweet Snap and Wando (a more susceptible cultivar) formed tumors that were intermediate in weight between the two parents.
Degree ProgramGraduate College