Microcosm experiments to enhance the bioremediation of a No.2 fuel oil-contaminated soil
AuthorRunyon, Thomas Alvin, 1963-
AdvisorArnold, Robert G.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractSoil microcosm experiments were conducted to identify soil amendments which enhance the biodegradation of a No. 2 (diesel) fuel in soil. Microcosm amendments in Phase 1 included yeast extract, acetate, methane, and activated sludge. Combinations of these amendments resulted in 16 different treatments. Phase 2 soil microcosms contained combinations of methane and methanotroph additions resulting in four different treatments. Gas chromatography was used to determine the time and treatment-dependent concentration of C12-C18 n-alkanes during Phase 1 and, C14-C19 n-alkanes during Phase 2. Results from Phase 1 indicated that the most extensive biodegradation occurred in methane and activated sludge-supplemented microcosms. Acetate and/or yeast extract inhibited biodegradation by soil and/or activated sludge microorganisms. Addition of methane relieved inhibition by these amendments. Methane and/or methanotroph additions to Phase 2 microcosms did not enhance biodegradation relative to unamended microcosms. In general, n-alkanes longer than n-C12 were equally degraded in soil microcosms.
Degree ProgramGraduate College