Evaluation of immunochemical assays for on-site determination of aflatoxin in cottonseed
AuthorNjapau, Henry, 1956-
AdvisorPark, Douglas L.
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractThe performance of four immunochemical test kits in detecting aflatoxin contamination in cottonseed under non-laboratory conditions was evaluated. Naturally contaminated cottonseed was analyzed at cotton gins, ammoniation plants, cotton oil processing plants, a dairy farm and at the University of Arizona. Evaluation was based on the ability to determine aflatoxin concentrations as more or less than 20 ppb when analyzed on-site and in the laboratory using a common extract. Comparison of results from both locations showed excellent agreement (Afla-20 Cup 86 ± 6%, (n = 188); Agri-Screen I, 75 ± 9%, (n = 56); Agri-Screen II, 93 ± 5%, (n = 106); Agri-Chek, 93 ± 4%, (n = 156) and Aflatest-10, 90 ± 4%, (n = 178)). Overall agreement between on-site and laboratory results was 88%. Comparison of kit results to those of TLC demonstrated a low potential for obtaining false negative values. From these results the kits can reliably be used to screen cottonseed for aflatoxin contamination under non-laboratory conditions.
Degree ProgramGraduate College
Nutrition and Food Sciences