Sequence analysis of a follicle cell-specific gene from the mosquito, Aedes aegypti
AuthorLin, Yonggu, 1963-
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PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractThe follicle cell-specific (FCS) gene, a 3,023 bp gene specific to follicle cells of the mosquito, Aedes aegypti, was characterized at the nucleotide level. Genomic Southern blots demonstrated that there was only one copy of this gene in the A. aegypti genome. An ovary-specific cDNA library was constructed from female mosquitoes 24 hours post blood meal. Then a cDNA clone containing the complete coding region was identified, and its nucleotide sequence was determined. The deduced protein contained unusually high levels of alanine and proline. Search of a protein data base revealed that the FCS gene was similar to Drosophila vitelline membrane protein genes, with 47.5% similarity in nucleotide sequence and 46.7% similarity in amino acid sequence. The conserved hydrophobic regions from several vitelline membrane proteins were compared. Another cDNA clone, 1D, was isolated from the cDNA library screened with the FCS gene. These two mosquito genes shared a 60% similarity at the nucleic acid level and a 79.8% similarity at the amino acid level.
Degree ProgramGraduate College