Inactivation of bacteriophages MS-2 and PRD1 and poliovirus type 1 in Pima Clay Loam and Brazito Sandy Loam soils amended with anaerobically digested sewage sludge
| dc.contributor.advisor | Gerba, Charles P. | en_US |
| dc.contributor.author | Straub, Timothy Mark, 1963- | |
| dc.creator | Straub, Timothy Mark, 1963- | en_US |
| dc.date.accessioned | 2013-04-03T13:05:08Z | |
| dc.date.available | 2013-04-03T13:05:08Z | |
| dc.date.issued | 1991 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10150/277884 | |
| dc.description.abstract | Inactivation of bacteriophages MS-2 and PRD1, and poliovirus type 1 were measured in Pima Clay Loam and Brazito Sandy Loam soils amended with anaerobically digested sewage sludge. Inactivation was determined at 15°C, 27°C, and 40°C with soils maintained at 30% moisture, and amended soils exposed to evaporation. No inactivation of PRD1 was observed within 30 days and 16 days at 15°C and 27°C respectively with little inactivation after 7 days at 40°C. Inactivation of MS-2 and poliovirus was approximately 2 fold greater at 27°C compared to 16°C. At 40°C neither virus was recovered 24 hours after sludge amendment. Evaporation to less than 5% soil moisture resulted in rapid loss of titer for all three viruses regardless of temperature. Survival of MS-2 and poliovirus 1 in sludge amended fields (15°C) was longer than comparable laboratory studies. None of the viruses were recovered in fields amended during the summer (33°C) after 7 days. A method was also developed to remove cell culture toxic components from these soils. | |
| dc.language.iso | en_US | en_US |
| dc.publisher | The University of Arizona. | en_US |
| dc.rights | Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. | en_US |
| dc.subject | Agriculture, Food Science and Technology. | en_US |
| dc.subject | Biology, Microbiology. | en_US |
| dc.subject | Health Sciences, Public Health. | en_US |
| dc.title | Inactivation of bacteriophages MS-2 and PRD1 and poliovirus type 1 in Pima Clay Loam and Brazito Sandy Loam soils amended with anaerobically digested sewage sludge | en_US |
| dc.type | text | en_US |
| dc.type | Thesis-Reproduction (electronic) | en_US |
| thesis.degree.grantor | University of Arizona | en_US |
| thesis.degree.level | masters | en_US |
| dc.identifier.proquest | 1343829 | en_US |
| thesis.degree.discipline | Graduate College | en_US |
| thesis.degree.discipline | Nutrition and Food Sciences | en_US |
| thesis.degree.name | M.S. | en_US |
| dc.identifier.bibrecord | .b26882607 | en_US |
| refterms.dateFOA | 2018-07-06T09:26:29Z | |
| html.description.abstract | Inactivation of bacteriophages MS-2 and PRD1, and poliovirus type 1 were measured in Pima Clay Loam and Brazito Sandy Loam soils amended with anaerobically digested sewage sludge. Inactivation was determined at 15°C, 27°C, and 40°C with soils maintained at 30% moisture, and amended soils exposed to evaporation. No inactivation of PRD1 was observed within 30 days and 16 days at 15°C and 27°C respectively with little inactivation after 7 days at 40°C. Inactivation of MS-2 and poliovirus was approximately 2 fold greater at 27°C compared to 16°C. At 40°C neither virus was recovered 24 hours after sludge amendment. Evaporation to less than 5% soil moisture resulted in rapid loss of titer for all three viruses regardless of temperature. Survival of MS-2 and poliovirus 1 in sludge amended fields (15°C) was longer than comparable laboratory studies. None of the viruses were recovered in fields amended during the summer (33°C) after 7 days. A method was also developed to remove cell culture toxic components from these soils. |
