Plasma protein binding and blood to plasma partitioning studies of methamphetamine
Publisher
The University of Arizona.Rights
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.Abstract
Methamphetamine is a sympathomimetic drug with CNS, cardiovascular and anorectic effects. We examined the Blood to Plasma (Blood/Plasma) partitioning and plasma protein binding (PB) of d-Methamphetamine (d-MAP), using whole rat blood. The mean Blood/Plasma ratio was around 1.2 and the fraction unbound (fᵤ) was 0.8. Further, we studied the influence of concentration, pH and the presence of 1-MAP on the Blood/Plasma ratio and PB of d-MAP. There was no significant change in the Blood/Plasma ratio and the PB values at different concentrations of d-MAP or in the presence of 1-MAP. There was a slight increase in the Blood/Plasma ratio and a slight but insignificant decrease in fᵤ with pH. The equilibrium binding constants (KA) of d-MAP with human serum albumin and α₁ -acid glycoprotein were also determined and they were found to be 213 and 2461 M⁻¹ respectively.Type
textThesis-Reproduction (electronic)
Degree Name
M.S.Degree Level
mastersDegree Program
Graduate CollegePharmaceutical Sciences