Theoretical and experimental studies of the plasma protein binding of high affinity binding drugs
| dc.contributor.advisor | Mayersohn, Michael | en_US |
| dc.contributor.author | Lee, Hui-Chih, 1963- | |
| dc.creator | Lee, Hui-Chih, 1963- | en_US |
| dc.date.accessioned | 2013-04-03T13:07:33Z | |
| dc.date.available | 2013-04-03T13:07:33Z | |
| dc.date.issued | 1991 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10150/277955 | |
| dc.description.abstract | A disadvantage of traditional equilibrium dialysis for highly protein bound drugs is the analytically low drug concentration found on the buffer side. We propose to replace a certain percentage of buffer with plasma containing drug in order to increase the total drug concentration on the buffer side. Computer simulations were performed to examine the effects of the percentage of plasma replacement of the buffer upon the increase of the total drug concentration on the buffer side after equilibrium dialysis. Further simulation results indicated that the development of a concise equation estimating the drug's equilibrium association binding constant (Ka) was feasible. Two high binding drugs, diazepam and nortriptyline, were examined to verify the advantages offered by the new proposed method and their Ka values were computed using the experimental results and the mathematical equation developed. The resulting data agreed well with theoretical predictions. | |
| dc.language.iso | en_US | en_US |
| dc.publisher | The University of Arizona. | en_US |
| dc.rights | Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author. | en_US |
| dc.subject | Biology, Molecular. | en_US |
| dc.subject | Chemistry, Pharmaceutical. | en_US |
| dc.subject | Health Sciences, Pharmacy. | en_US |
| dc.title | Theoretical and experimental studies of the plasma protein binding of high affinity binding drugs | en_US |
| dc.type | text | en_US |
| dc.type | Thesis-Reproduction (electronic) | en_US |
| thesis.degree.grantor | University of Arizona | en_US |
| thesis.degree.level | masters | en_US |
| dc.identifier.proquest | 1345433 | en_US |
| thesis.degree.discipline | Graduate College | en_US |
| thesis.degree.discipline | Pharmaceutical Sciences | en_US |
| thesis.degree.name | M.S. | en_US |
| dc.identifier.bibrecord | .b27031093 | en_US |
| refterms.dateFOA | 2018-08-27T12:15:57Z | |
| html.description.abstract | A disadvantage of traditional equilibrium dialysis for highly protein bound drugs is the analytically low drug concentration found on the buffer side. We propose to replace a certain percentage of buffer with plasma containing drug in order to increase the total drug concentration on the buffer side. Computer simulations were performed to examine the effects of the percentage of plasma replacement of the buffer upon the increase of the total drug concentration on the buffer side after equilibrium dialysis. Further simulation results indicated that the development of a concise equation estimating the drug's equilibrium association binding constant (Ka) was feasible. Two high binding drugs, diazepam and nortriptyline, were examined to verify the advantages offered by the new proposed method and their Ka values were computed using the experimental results and the mathematical equation developed. The resulting data agreed well with theoretical predictions. |
