Methods development for the in vitro assessment of cutaneous drug metabolism
AuthorThohan, Sanjeev, 1962-
KeywordsHealth Sciences, Pharmacology.
AdvisorSipes, I. Glenn
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractThe major objective of this work was development of procedures for the quantification of cutaneous microsomal cytochrome P-450 mediated metabolism using model substrates. The Fischer-344 rat was the species of choice for this developmental work. The versatility of this method was demonstrated through its application to a variety of species including man. An extension of the rat microsomal work entailed the use of inducing agents, specifically, the Aroclor series of polychlorinated biphenyls. The effects of these inducing agents were demonstrated and characterized in skin and liver with respect to microsomal marker enzymes and substrate metabolism. In addition to the microsomal studies, the use of rat skin explants in culture, provided a simple system for the characterization of phase I and phase II coupled metabolism of the model substrates.
Degree ProgramGraduate College
Pharmacology & Toxicology