Use of staphylococcal enterotoxin A-induced interleukin 2 production as an indicator of immunotoxicity
AuthorReid, Lynnda Louise, 1949-
AdvisorGandolfi, A. Jay
MetadataShow full item record
PublisherThe University of Arizona.
RightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
AbstractAn in vitro assay measuring IL-2 production as an index of compromised immune function was developed using primary splenocytes from female C57BL/6 mice pretreated with olive oil, 30 mg/kg Cyclosporin A, or 220-880 mg/kg benzene. Splenocytes were mitogen-stimulated to produce IL-2 with SEA, Con A or PHA. Benzene induced inhibition of IL-2 production was demonstrated by incubating the splenocyte culture supernatant fraction with an IL-2 dependent T-lymphocytic cell line. Cell proliferation was measured by blastogenesis and MTT reduction as an index of IL-2 mediated response. Benzene and cyclosporin dosed animals demonstrated a significant decrease in splenocyte proliferation. However, cell proliferation in IL-2 dependent cells was inhibited only with the higher doses of benzene indicative of a dose dependent IL- 2 mediated suppression. Chemically induced impairment of IL-2 mediated immune function may be detected with this assay.