• Login
    View Item 
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    L-type calcium channels mediate nicotinic acetylcholine receptor aggregation on cultured myotubes

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    azu_td_3107021_sip1_m.pdf
    Size:
    3.344Mb
    Format:
    PDF
    Download
    Author
    Milholland, Rebecca
    Issue Date
    2003
    Keywords
    Biology, Molecular.
    Biology, Neuroscience.
    Advisor
    Gordon, Herman
    Yool, Andrea
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    In this dissertation, I have presented new information on several aspects of the signaling pathway responsible for the clustering of AChRs on muscle cells. First, I have shown that activation of L-CaChs is both necessary for agrin induced clustering of AChRs and sufficient to stimulate AChR clustering even in the absence of agrin. Additionally, I have shown that activation of AChRs causes their own clustering by influencing the activity of L-CaChs. I have also shown that neither AChRs nor L-CaChs play a role in MuSK activation or AChR beta subunit phosphorylation suggesting that the role of AChR and L-CaCh is downstream of MuSK activation and phosphorylation of the AChR beta subunit in the signaling cascade that leads to the aggregation of AChRs. Finally, I have shown that calcium induced clustering and phosphorylation of AChRs require LCaCh activation. These data suggested that although L-CaCh activation is insufficient to cause AChR beta subunit phosphorylation L-CaCh may modulate an intermediate step between MuSK activation and AChR phosphorylation. These data therefore support the hypothesis that L-CaCh activation delivers extracellular calcium to the intracellular machinery that regulates AChR clustering. Furthermore, these data establish the position of L-CaChs in the signaling hierarchy responsible for AChR clustering as being downstream of or parallel to both MuSK activation and AChR phosphorylation in the signaling cascade behind AChR clustering. The data presented in this paper begin to provide an integrated view of NMJ formation in which neuromuscular transmission, calcium signaling, and signaling cascades mediated by neurotrophic factors act in concert to regulate the localization of synaptic molecules to junctional regions of the muscle fiber. Many questions remain, however, regarding the events downstream of MuSK and L-CaCh activation.
    Type
    text
    Dissertation-Reproduction (electronic)
    Degree Name
    Ph.D.
    Degree Level
    doctoral
    Degree Program
    Graduate College
    Pharmacology & Toxicology
    Degree Grantor
    University of Arizona
    Collections
    Dissertations

    entitlement

     
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.