• Login
    View Item 
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA Catalogs

    Statistics

    Display statistics

    THERMAL INJURY OF YERSINIA ENTEROCOLITICA

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    azu_td_8017809_sip1_m.pdf
    Size:
    1.098Mb
    Format:
    PDF
    Download
    Author
    Restaino, Lawrence
    Issue Date
    1980
    Keywords
    Yersinia enterocolitica.
    Advisor
    Jeter, Wayburn S.
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    Procedures were developed to evaluate thermal injury to three strains of Yersinia enterocolitica (serotype 0:3, 0:8 and 0:17). Serotype 0:17 (atypical strain) was more sensitive to bile salts (BS) #3 and to sublethal heat treatment than the typical strains, 0:3 and 0:8. When the 0:3, 0:8 and 0:17 serotypes were thermally stressed in 0.1 M PO₄ buffer, pH=7.0, at 47C for 70, 60 and 12 min, respectively, greater than 99% of the total viable cell population was injured. Injury was determined by the ability of cells to form colonies on Brain heart infusion (BHI) agar, but not on Trypticase soy agar (TSA) plus 0.6% BS #3 for serotypes 0:3 and 0:8 and TSA plus 0.16% BS #3 for 0:17. Heat injury of serotype 0:17 cells for 15 min in 0.1 M PO₄ buffer caused an approximate 1000-fold reduction in cell numbers on selective media as compared to cells heated in PI, BHI broth and 10% nonfat dry milk (NFDM). The extended lag and resuscitation period on BHI broth was 2.5 times greater for 0:17 cells injured in 0.1 M PO₄ than for cells injured in BHI or PI menstruums. The rate and extent of repair of Y. enterocolitica 0:17 cells in three recovery media were directly related to the heating menstruum used for injury. The use of metabolic inhibitors demonstrated that ribonucleic acid (RNA) synthesis was required for repair, whereas deoxyribonucleic (DNA), cell wall and protein synthesis were not necessary for recovery of 0:17 cells injured in 0.1 M PO₄ buffer, BHI or PI menstruums. Inhibition of respiration by 2,4-dinitrophenol slowed repair only for 0:17 cells injured in 0.1 M PO₄ buffer, but not cells injured in PI or BHI menstruums.
    Type
    text
    Dissertation-Reproduction (electronic)
    Degree Name
    Ph.D.
    Degree Level
    doctoral
    Degree Program
    Graduate College
    Microbiology and Medical Technology
    Degree Grantor
    University of Arizona
    Collections
    Dissertations

    entitlement

     
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.