• Login
    View Item 
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    •   Home
    • UA Graduate and Undergraduate Research
    • UA Theses and Dissertations
    • Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UA Campus RepositoryCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournalThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsPublisherJournal

    My Account

    LoginRegister

    About

    AboutUA Faculty PublicationsUA DissertationsUA Master's ThesesUA Honors ThesesUA PressUA YearbooksUA CatalogsUA Libraries

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Investigations into new approaches for analyzing pharmaceuticals through the use of array detector imaging of high-performance TLC and well plates

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    azu_td_9946830_sip1_c.pdf
    Size:
    26.17Mb
    Format:
    PDF
    Download
    Author
    Simon, Richard Edward
    Issue Date
    1999
    Keywords
    Chemistry, Analytical.
    Chemistry, Pharmaceutical.
    Advisor
    Denton, M. Bonner
    
    Metadata
    Show full item record
    Publisher
    The University of Arizona.
    Rights
    Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
    Abstract
    New methods of analyzing pharmaceuticals by high performance thin-layer chromatography and microplate imaging were investigated using array imaging technology. Both techniques provide high sample throughput over more traditional analytical techniques for the analysis of pharmaceuticals. HPTLC provides high sample throughput by performing separations in parallel using a planar stationary phase. Imaging the entire plate with a single exposure performs quantitation of the analytes in the chromatographic medium. Fluorescence and fluorescence quenching detection modes are presented by employing tetracyclines, famotidine, and several over the counter drugs as model compounds. Studies conducted include sensitivity, separation efficiency and reproducibility of the system. Microplate imaging allows for the quantitation of numerous analytes in parallel. In this technique, solutions containing the analyte of interest are deposited into numerous self-contained wells on microplates, also known as 96-wellplates or ELISA plates. Light is passed through the wells of the plate allowing for absorption, fluorescence, or fluorescence quenching. Imaging with an array detector enables the researcher to simultaneously quantitate each well in parallel. The reaction between primary amines, ampicillin and amoxicillin, and fluorescamine was investigated. Microplate imaging was also tested for quantitating analytes in the low UV region (254 nm).
    Type
    text
    Dissertation-Reproduction (electronic)
    Degree Name
    Ph.D.
    Degree Level
    doctoral
    Degree Program
    Graduate College
    Chemistry
    Degree Grantor
    University of Arizona
    Collections
    Dissertations

    entitlement

     
    The University of Arizona Libraries | 1510 E. University Blvd. | Tucson, AZ 85721-0055
    Tel 520-621-6442 | repository@u.library.arizona.edu
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.